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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Flow Cytometry, CD34

Test Code(s): ABCD34 / HCABCD34

CPT Code(s): 86367

Test Description: Test Component:

Percent and absolute number of CD34 cells in peripheral blood


Methodology: Flow Cytometry

Clinical Significance: For determining CD34 level in peripheral blood prior to apheresis collection.

Days Performed: Mon-Fri, dayshift.

Turnaround Time: Routine: 1 working day.


Specimen Requirements

Specimen: Blood

Collection Instructions: An order for a WBC and differential must accompany request.

Outside clients should send WBC and differential results along with specimen. If no results accompany request, a WBC and differential will be added (see transport and limitations for more information).

Collection Container: Preferred: Lavender top

Collection Volume: Preferred: 4 mL
Pediatrics: 1 mL

Sample Analyzed: Whole Blood

Volume Required: Preferred: 1 mL
Pediatrics: 0.5 mL

Specimen Processing: Keep specimen at room temperature. Do NOT centrifuge tube.

Specimen Transport: Transport unopened tube at room temperature to UWHC Core Laboratory (B4/220) within 6 hours of collection. Maintain ambient temperature during transport. If sending WBC and differential results, specimen must be received within 8 hours of collection.

Unacceptable Criteria: Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Samples greater than 24 hours old at time of analysis are not acceptable.

Stability: Ambient: 6 hours
Refrigerated: Not acceptable
Frozen: Not acceptable


Interpretation

Additional Information:

This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

Flow Cytometry - CD34 on Peripheral blood Apheresis Product

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