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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Flow Cytometry, Immune Deficiency Profile

Test Code(s): PBPLUS / HCPBPLUS, FPPLUS / HCFPPLUS (if WBC and Differential not ordered)

CPT Code(s): 86359,86355,86360,86357,86356x3

Test Description: Test Component:

Absolute lymphocyte count, total T-cell, B-cell and NK-cell quantitation, CD4 and CD8 subset analysis, CD4:CD8, CD4+CD45RA+, CD4+CD45RO+, CD4+CD45RA+CD45RO+


Methodology: Flow Cytometry

Days Performed: Mon-Fri, dayshift- specimens accepted at anytime.

Turnaround Time: Stat: Not available stat.
Routine: 1 - 4 days.


Specimen Requirements

Specimen: Blood

Collection Instructions: An order for a WBC and differential must accompany request.

Outside clients should send WBC and differential results along with specimen. If no results accompany request, a WBC and differential will be added (see transport and limitations for more information).

Collection Container: Preferred: Lavender top

Collection Volume: Preferred: 4 mL
Pediatrics: 1 mL

Sample Analyzed: Whole Blood

Volume Required: Preferred: 1 mL
Pediatrics: 0.5 mL

Specimen Processing: Keep specimen at room temperature. Do NOT centrifuge tube.

Specimen Transport: Transport unopened tube at room temperature to UWHC Core Laboratory (B4/220) within 4 hours of collection. Maintain ambient temperature during transport. For outreach locations sending WBC and differential results along with specimen, specimen may be received within 8 hours of collection.

Unacceptable Criteria: Hemolyzed, clotted, frozen or overheated specimens are not acceptable.  Samples greater than 24 hours old at time of analysis are not acceptable.

Stability: Ambient: 6 hours if WBC and Differential results do not accompany specimen, 24 hours if WBC and Differential results accompany specimen.
Refrigerated: Not acceptable
Frozen: Not acceptable


Interpretation

Reference Interval:

Age 18 and up:

   %   Absolute #/uL
     
CD3  59-85  767-1903
CD3+CD4+  37-64  494-1464
CD3+CD8+  14-33  247-595
CD3-CD19+  6-24  88-454

CD3-CD16+

CD56+

 6-24  77-427

CD3+CD16+

CD56+

 1-13  15-190
     
CD4+CD45RA+

reference interval

not established

reference interval

not established

CD4+CD45RO+

reference interval

 not established

reference interval

not established

CD4+CD45RA+CD45RO+

reference interval

not establised

reference interval

not established

CD4:CD8  1.2-3.7  

 

Please contact UWHC Flow Laboratory at (608) 263-3868 for

pediatric reference intervals.

Test Limitations:

Absolute values will not be calculated if WBC and differential request or results are not received.

Additional Information:

This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

Flow Cytometry Extended Immune Deficiency Panel lymphocytes T B and NK-cell Quantitation Analysis T-Cell B-Cell Primary Immunodeficiency Profile

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