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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Flow Cytometry, CD3

Test Code(s): TOT3 / HCTOT3

CPT Code(s): 86359

Test Description: Test Component:

Absolute lymphocyte count, percent and absolute numbers of CD3 cells (T-cells)


Methodology: Flow Cytometry

Clinical Significance: CD3 analysis is used to follow CD3 level post certain immune suppressive therapies (eg. OKT3, ATG).

Days Performed: Mon-Fri, dayshift- specimens accepted at anytime.

Turnaround Time: Stat: Not available stat.
Routine: 1 - 4 days.


Specimen Requirements

Specimen: Blood

Collection Instructions: An order for a WBC and differential must accompany request.  Outside clients should send WBC and differential results along with specimen. If no results accompany request, a WBC and differential will be added.

Collection Container: Preferred: Lavender top

Collection Volume: Preferred: 4 mL
Pediatrics: 1 mL

Sample Analyzed: Whole Blood

Volume Required: Preferred: 1 mL
Pediatrics: 0.5 mL

Specimen Processing: Keep specimen at room temperature. Do NOT centrifuge tube.

Specimen Transport: Transport unopened tube at room temperature to UWHC Core Laboratory (B4/220) within 4 hours of collection. Maintain ambient temperature during transport. DO NOT centrifuge.

Unacceptable Criteria: Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Samples greater than 24 hours old at time of analysis are not acceptable.

Stability: Ambient: 6 hours if WBC and Differential results do not accompany specimen, 24 hours if WBC and Differential results accompany specimen
Refrigerated: Not acceptable
Frozen: Not acceptable


Interpretation

Test Limitations:

Absolute values will not be calculated if WBC and differential request or results are not received.

Additional Information:

This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

Flow Cytometry CD3 Panel Quantitation

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