| Test Name: |
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Flow Cytometry, T Cell Analysis
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| Test Code(s): |
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TCA / HCTCA
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| CPT Code(s): |
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86359, 86360, 86355, 86357
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| Test Description: |
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Test Component: Absolute lymphocyte count, percent and absolute numbers of CD3 (T-cells), CD3 CD4 (T-helper cells), CD3 CD8 (T-cytotoxic/suppressor cells), CD3-CD19 (B-cells), CD3-CD16 CD56 (NK-cells), CD4:CD8
(Note: Absolute lymphocyte count and absolute numbers will only be done on blood specimens)
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| Methodology: |
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Flow Cytometry
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| Days Performed: |
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Mon-Fri, dayshift- specimens accepted at anytime.
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| Turnaround Time: |
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Routine: Approximately 2 working days.
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Specimen Requirements
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| Specimen: |
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See collection instructions.
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| Collection Instructions: |
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An order for a WBC and differential must accompany blood specimen requests. Outside clients should send WBC and differential results along with specimen. If no results accompany request, a WBC and differential will be added (see Transport, Limitations and Additional Information for more information).
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| Collection Container: |
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Preferred: See collection instructions.
Also Acceptable:
See collection instructions.
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| Specimen Processing: |
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Blood specimens: Keep specimen at room temperature. Do NOT centrifuge tube.
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| Specimen Transport: |
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Transport BLOOD specimens to UWHC Core Laboratory (B4/220) within 6 hours of collection.
Transport BAL specimens to UWHC Core Laboratory (B4/220) within one hour of collection if a Body Fluid cell count is ordered.
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| Outreach Specimen Transport: |
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For BLOOD specimens transport unopened tube at room temperature to UWHC Core Laboratory (B4/220) within 6 hours of collection if the WBC and Differential results do not accompany the specimen.
If sending WBC and Differential results, specimen must be received within 22 hours of collection.
For BAL specimens that have a body fluid cell count ordered the specimen must be transported to the UWHC Core Laboratory (B4/220) within one hour of collection.
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| Unacceptable Criteria: |
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Blood samples that are hemolyzed, clotted, frozen or overheated specimens are not acceptable. Blood samples greater than 24 hours old at time of analysis are not acceptable.
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| Stability: |
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Ambient: See additional information
Refrigerated: See additional information
Frozen: Not Acceptable
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Interpretation
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Reference Interval:
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For Blood specimens only:
Age 18 and up:
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% |
Absolute number/uL |
| CD3: |
59-85 |
767-1903 |
| CD3+CD4+: |
37-64 |
494-1464 |
| CD3+CD8+: |
14-33 |
247-595 |
| CD3-CD19+: |
6-24 |
88-454 |
| CD3-CD16+CD56+: |
6-24 |
77-427 |
| CD4:CD8 ratio: |
1.2-3.7 |
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Please contact UWHC Flow Laboratory at (608) 263-3868 for blood pediatric reference intervals.
No reference ranges for Bronchoalveolar lavage specimens.
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| Test Limitations: |
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Absolute values will not be calculated for blood specimens if WBC and differential request or results are not received.
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| Additional Information: |
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Blood Specimens:
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Container
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Volume |
Stability |
Transport |
| Lavender |
Preferred: 4 mL
Min. 1 mL
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Ambient: 8 hours
if CBC & Diff
is needed.
Ambient: 24 hours
if CBC & Diff is not needed.
Refrigerate: Not
Acceptable
Frozen- Not Acceptable
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Transport within 6 hr if cell count is needed
Transport within 22 hr if cell count is not needed
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BAL Specimens:
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Container
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Volume |
Stability |
Transport |
| Sterile Container |
Min. 1 mL
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Ambient: 2 hours
if CBC & Diff is needed.
Ambient: 24 hours
if CBC & Diff is not needed.
Refrigerate: Not
Acceptable
Frozen- Not Acceptable
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Transport within 1 hr if cell count is needed
Transport within 22 hr if cell count is not needed
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This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.
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