UW Hospital and Clinics Lab Test Directory
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Cytology, ThinPrep Pap Test
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| Test Code(s): |
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HCPATHP
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| CPT Code(s): |
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88142
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| Clinical Significance: |
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Compared to a conventional Pap smear, the ThinPrep Pap Test has demonstrated increased sensitivity and specificity for detection of low grade and high grade squamous intra-epithelial lesions. Specimen adequacy has also improved with the ThinPrep Pap Test.
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| Request Form: |
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Cytopathology (UWH 1280520A)
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| Days Performed: |
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Mon-Fri, 0800-1700.
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| Turnaround Time: |
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Routine: 3 working days.
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Specimen Requirements
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| Specimen: |
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See collection instructions.
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| Patient Preparation: |
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Avoid vaginal medications, vaginal lubricants, vaginal contraceptives, or douches 48 hours prior to the Pap Test.
Do not schedule a Pap Test during menses.
Perform a visual exam of the cervix. If excess mucus or other discharge is present, gently remove with a dry gauze pad prior to sample collection.
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| Collection Instructions: |
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SPATULA/ENDOCERVICAL BRUSH PROTOCOL:
1. Rotate plastic spatula at least 1 full turn around the cervical os and ectocervix.
2. Quickly rinse the spatula in the PreservCyt vial. Swirl vigorously 10 times. Discard the spatula.
3. Insert the brush into the os until just the bottom bristles are exposed. Slowly rotate 1/4 to 1/2 turn in one direction only. DO NOT OVER ROTATE.
4. Quickly rinse the brush by rotating and pushing it against the vial wall 10 times; vigorously swirl the brush again. Discard the brush.
ALTERNATIVE BROOM-LIKE DEVICE PROTOCOL:
1. Insert the central bristles into the endocervical canal; allow the shorter bristles to fully contact the ectocervix.
2. Push gently and fully rotate the broom CLOCKWISE 3-5 times.
3. Quickly rinse the broom in the vial. Push the broom against the bottom of the vial 10 times. Swirl again vigorously.
4. Discard the entire device.
For both protocols, tighten the vial cap so the torque line on the cap passes the torque line on the vial. Label the vial with a pre-printed patient label.
NOTE: DO NOT LEAVE THE COLLECTION DEVICE IN THE VIAL.
NOTE: DO NOT WRAP THE VIAL CAP WITH PARAFILM.
See Additional Information field to view video on ThinPrep Pap Collection.
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| Collection Container: |
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Preferred: ThinPrep PreservCyt Solution Vial 20 mL
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| Collection Volume: |
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Preferred: 20 mL
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| Specimen Transport: |
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Place specimen inside a biohazard bag with the request form in the outside pocket of the bag. Transport specimen to UWHC Core Laboratory (B4/220).
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| Unacceptable Criteria: |
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Failure to tighten the cap on the PreservCyt vial may cause loss of the specimen.
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| Stability: |
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Ambient: 3 weeks
Frozen: Not Acceptable
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Interpretation
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Report provides interpretation. Please call the UWHC Cytology Lab at (608) 263-3205 for further information.
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| Test Limitations: |
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Over rotation of the endocervical brush does not increase cell yield, but may cause bleeding. Abnormal findings must be correlated with history and other test results.
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| Additional Information: |
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Paperwork must include patient name, medical record number, birth date, specimen source, collection date, clinic and phone number, clinicia's full name and pager number, pertinent clinical history, LMP date and results of previous Pap test.
Refer to ThinPrep Pap Collection video for collection information.
Refer to Pap and Thin Prep Testing FAQ's for answers on more frequently asked questions.
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Pap Smear, Cervical, Vaginal, Thinprep
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