| Test Name: |
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Direct Antiglobulin Test
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| Test Code(s): |
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DAT / DCMB
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| CPT Code(s): |
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86880
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| Methodology: |
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Hemagglutination
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| Days Performed: |
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Daily, 24 hours.
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| Turnaround Time: |
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Stat: 1 hour.
Routine: 8 hours.
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Specimen Requirements
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| Specimen: |
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Blood
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| Collection Instructions: |
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Indicate collection date and time on specimen.
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| Collection Container: |
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Preferred: Lavender top
Also Acceptable:
Microtainer,
Pink top tube
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| Collection Volume: |
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Preferred: 4 mL
Pediatrics: 0.5 mL
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| Sample Analyzed: |
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Red cells
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| Specimen Transport: |
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Transport specimen to UWHC Transfusion Service (C7/381) or send to tube station 134.
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| Unacceptable Criteria: |
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Clotted specimens or are not acceptable.
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| Stability: |
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Ambient: 24 hours
Refrigerated: 24 hours
Frozen: Not acceptable
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Interpretation
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Expected Results:
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Negative
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| Additional Information: |
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All red cells found to be positive with polyspecific Coombs' serum will be further tested with monospecific Coombs' sera (anti-IgG and anti-complement).
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