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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Direct Antiglobulin Test

Test Code(s): DAT / DCMB

CPT Code(s): 86880

Methodology: Hemagglutination

Days Performed: Daily, 24 hours.

Turnaround Time: Stat: 1 hour.
Routine: 8 hours.


Specimen Requirements

Specimen: Blood

Collection Instructions: Indicate collection date and time on specimen.

Collection Container: Preferred: Lavender top
Also Acceptable: Microtainer, Pink top tube

Collection Volume: Preferred: 4 mL
Pediatrics: 0.5 mL

Sample Analyzed: Red cells

Specimen Transport: Transport specimen to UWHC Transfusion Service (C7/381) or send to tube station 134.

Unacceptable Criteria: Clotted specimens are not acceptable.

Stability: Ambient: 24 hours
Refrigerated: 24 hours
Frozen: Not acceptable


Interpretation

Expected Results:

Negative

Additional Information:

All red cells found to be positive with polyspecific Coombs' serum will be further tested with monospecific Coombs' sera (anti-IgG and anti-complement).

Direct Coombs

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