| Test Name: |
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Sirolimus
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| Test Code(s): |
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SIRO / HCSIRO
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| CPT Code(s): |
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80195
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| Methodology: |
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Liquid Chromatography/Mass Spectrometry
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| Clinical Significance: |
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Immunosuppressive
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| Days Performed: |
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Daily, dayshift- Pathology Resident approval is required after hours.
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| Turnaround Time: |
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Routine: 24 hours.
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Specimen Requirements
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| Specimen: |
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Blood
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| Patient Preparation: |
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Sirolimus level should be determined on trough specimen collected just prior to administration of the next dose.
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| Collection Instructions: |
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Collect specimen just before taking next dose.
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| Collection Container: |
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Preferred: Lavender top
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| Collection Volume: |
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Preferred: 4 mL
Pediatrics: 0.5 mL
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| Sample Analyzed: |
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Whole Blood
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| Specimen Processing: |
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Do NOT centrifuge or separate plasma. SEND WHOLE BLOOD. Refrigerate.
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| Specimen Transport: |
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Transport specimen at ambient temperature to UWHC Core Laboratory (B4/220). Do NOT freeze. SPECIMEN MUST ARRIVE WITHIN 5 DAYS OF COLLECTION.
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| Stability: |
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Ambient: 28 days
Refrigerated: 28 days
Frozen: 6 months
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Interpretation
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Interpretive Guidelines:
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A definitive therapeutic concentration for sirolimus has not been established. A concentration of 5-15 ng/mL is generally considered to be therapeutic for individuals 18 years old and up.
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| Critical Calls: |
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View
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| Additional Information: |
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The reportable range for sirolimus is 1 - 50 ng/mL. Results of less than 1 ng/ mL will be reported as less than 1 ng/mL.
This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.
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