| Test Name: |
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Pentobarbital
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| Test Code(s): |
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PENT / HCPENT
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| CPT Code(s): |
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82205
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| Methodology: |
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Gas Chromatography
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| Clinical Significance: |
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Hypnotic, sedative
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| Days Performed: |
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Daily, dayshift- Pathology Resident approval is required after hours.
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| Turnaround Time: |
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Stat: 3 hours.
Routine: 24 hours.
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Specimen Requirements
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| Specimen: |
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Blood
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| Collection Container: |
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Preferred: Red top
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| Collection Volume: |
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Preferred: 3 mL
Pediatrics: 1 mL
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| Sample Analyzed: |
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Serum
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| Volume Required: |
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Preferred: 1 mL
Pediatrics: 0.4 mL
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| Specimen Processing: |
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Centrifuge. Transfer cell-free serum to plastic vial. Refrigerate.
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| Specimen Transport: |
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Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.
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| Unacceptable Criteria: |
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Specimens collected in red cap with yellow ring (SST) tubes are not acceptable.
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Interpretation
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Interpretive Guidelines:
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Generally effective therapeutic concentration when used in treatment of increased intracranial pressure:
18 years and up: 2.5 - 4.0 mg/dL
All results greater than 3.0 mg/dL are considered critical and will be called to the ordering source.
NOTE: When thiopental is administered all pentobarbital results >1 mg/dL are considered critical and will be called to the ordering source.
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| Critical Calls: |
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View
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| Additional Information: |
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Result of "BELOW" indicates that the result is below the method detection limit of 0.2 mg/dL. The half-life is 15-48 hours. Peak serum concentration is usually reached 0.5-2 hours after a single oral dose.
This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.
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