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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Imipramine/ Desipramine

Test Code(s): IMI / XIDF

CPT Code(s): 80335

Test Description: Test Component:

Imipramine and desipramine

Methodology: Liquid Chromatography

Clinical Significance: Antidepressant

Days Performed: Daily, dayshift.

Turnaround Time: Routine: 24 hours.

Specimen Requirements

Specimen: Blood

Patient Preparation: The patient should be on the same dose for at least five days before collecting a specimen for therapeutic drug monitoring.

Collection Instructions: Collect specimen for trough level prior to next dose.

Collection Container: Preferred: Red top

Collection Volume: Preferred: 4 mL
Pediatrics: 4 mL

Sample Analyzed: Serum

Volume Required: Preferred: 2 mL
Pediatrics: 1.5 mL

Specimen Processing: Centrifuge. Transfer cell-free serum to plastic vial. Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Unacceptable Criteria: Specimens collected in red cap with yellow ring (SST) tubes are not acceptable.

Stability: Ambient: 5 days
Refrigerated: 7 days
Frozen: 1 year


Interpretive Guidelines:
Generally effective therapeutic concentration:  
18 years and up:

Imipramine plus

desipramine: 180-350 ng/mL

18 years and up:

Total tricyclic concentration

of >500 ng/mL  


Critical Calls: View

Additional Information:

Result of "BELOW" indicates that the result is below method detection limit of 10 ng/mL. The half-life of imipramine is 9-24 hours in an adult, and 6-15 hours in a child. The half-life of the metabolite, desipramine is 12-54 hours in an adult.


This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories.  This test meets the standards for clinical testing.  This test should not be regarded as investigational or for research use.  The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

Tofranil, tricyclic

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