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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Hemoglobin, Plasma

Test Code(s): PHGB / HCPHGB

CPT Code(s): 83051

Methodology: Colorimetric

Days Performed: Mon-Fri.

Turnaround Time: Stat: Not available stat.
Routine: Up to 4 days

Specimen Requirements

Specimen: Blood

Collection Instructions: Avoid mechanical trauma. See "Additional Information" below.

Collection Container: Preferred: Green top
Also Acceptable: Green cap with yellow ring (PST), Red top

Collection Volume: Preferred: 3 mL
Pediatrics: 1 mL

Sample Analyzed: Plasma or serum

Volume Required: Preferred: 1 mL
Pediatrics: 0.2 mL

Specimen Processing: Centrifuge. Transfer cell-free plasma/serum to plastic vial. Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Stability: Ambient: 2 days
Refrigerated: 2 days
Frozen: 6 months


Expected Results:

Plasma or serum from healthy individuals may contain up to 5 mg/dL hemoglobin.

Critical Calls: View

Additional Information:

Result of "BELOW" indicates that the result is below the method detection limit of 5 mg/dL. Mechanical trauma during the blood drawing process or excessive handling of the specimen should be avoided. Hemolysis may occur, which would invalidate the analytical results for the sample. If the plasma hemoglobin concentration is greater than 135 mg/dL, it may then be detected in urine. The quantitative analysis for the hemoglobin content of plasma or serum samples is identical and the reference range is the same. It is useful to test a plasma or serum sample for any patient with a suspected transfusion reaction. An elevated free hemoglobin in a patient specimen may indicate a hemolytic response.


This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories.  This test meets the standards for clinical testing.  This test should not be regarded as investigational or for research use.  The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

Free Hemoglobin Serum Hemoglobin HGB Plasma Hemoglobin PHGB

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