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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Caffeine

Test Code(s): CAFF / HCCAFF

CPT Code(s): 80155

Methodology: Gas Chromatography

Clinical Significance: Neonatal apnea

Days Performed: Daily, dayshift.

Turnaround Time: Stat: 3 hours.
Routine: 24 hours.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red top

Collection Volume: Preferred: 2 mL
Pediatrics: 1 mL

Sample Analyzed: Serum

Volume Required: Preferred: 1 mL
Pediatrics: 0.3 mL

Specimen Processing: Centrifuge. Transfer cell-free serum to plastic vial. Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Unacceptable Criteria: Specimens collected in red cap with yellow ring (SST) tubes are not acceptable.

Stability: Ambient: 7 days
Refrigerated: 7 days
Frozen: 1 year


Interpretive Guidelines:

Generally effective therapeutic

concentration for neonatal apnea: 

8.0-20.0 mcg/mL 
Potentially toxic:  over 50.0 mcg/mL 


Critical Calls: View

Additional Information:

Result of "BELOW" indicates that the result is below the method detection limit of 0.5 mcg/mL. Half-life of caffeine is 3-7 hours in adults, 41-230 hours in neonates.


This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories.  This test meets the standards for clinical testing.  This test should not be regarded as investigational or for research use.  The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

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