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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Heparin Induced Platelet Ab

Test Code(s): HEPAB / HCHEPAB

CPT Code(s): 86022

Methodology: ELISA

Clinical Significance: Test is used for the qualitative detection in plasma or serum of the anti-heparin-platelet factor 4 antibodies generated during type II heparin-induced thrombocytopenias.

Days Performed: Daily- Specimens are accepted at any time. Testing required during off hours must be approved by a Coagulation physician. Call (608)263-5005 before collection.

Turnaround Time: Stat: 1 day.
Routine: 2 days.


Specimen Requirements

Specimen: Blood

Collection Instructions: Please indicate if patient is receiving anticoagulant (Warfarin/Heparin/Vitamin K antagonist), direct thrombin inhibitor or fibrinolytic agent therapy.

Collection Container: Preferred: Light blue top (3.2% NaCitrate)
Also Acceptable: Red top

Collection Volume: Preferred: Full 3.5 mL tube
Pediatrics: Pediatric tube filled to fill mark

Sample Analyzed: Plasma or serum

Volume Required: Preferred: 1 mL
Pediatrics: 0.5 mL

Specimen Processing: Centrifuge specimen to yield platelet poor plasma/serum (platelet count should be less than 10 K/L). Separate plasma/serum and transfer to plastic tube or vial. Freeze plasma/serum at -20C or below.  

Specimen Transport: Transport whole blood specimen at room temperature to UWHC Core Laboratory (B4/220) within 4 hours of collection. See Specimen Processing for instructions if transport criteria are not met. Transport frozen aliquot on dry ice to UWHC Core Laboratory (B4/220).

Unacceptable Criteria: Hemolyzed specimens, clotted specimens, partially filled tubes and whole blood specimens greater than 4 hours old when received by the laboratory are not acceptable.

Stability: Ambient: 24 hours on a spun tube
Refrigerated: Not acceptable
Frozen: 2 weeks at -20C; 6 months at -70C


Interpretation

Expected Results:

0 days and up: Negative

Additional Information:

The optical density (OD) of the reference calibrator and the patient are reported as an aid for interpretaton.

HIT Antibodies Heparin Associated Platelet

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