UW Hospital and Clinics Lab Test Directory
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Heparin Induced Platelet Ab
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| Test Code(s): |
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HEPAB / HCHEPAB
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| CPT Code(s): |
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86022
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| Methodology: |
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ELISA
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| Clinical Significance: |
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Test is used for the qualitative detection in plasma or serum of the anti-heparin-platelet factor 4 antibodies generated during type II heparin-induced thrombocytopenias.
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| Days Performed: |
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Daily- Specimens are accepted at any time. Testing required during off hours must be approved by a Coagulation physician. Call (608)263-5005 before collection.
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| Turnaround Time: |
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Stat: 1 day.
Routine: 2 days.
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Specimen Requirements
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| Specimen: |
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Blood
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| Collection Instructions: |
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Please indicate if patient is receiving anticoagulant (Warfarin/Heparin/Vitamin K antagonist), direct thrombin inhibitor or fibrinolytic agent therapy.
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| Collection Container: |
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Preferred: Light blue top (3.2% NaCitrate)
Also Acceptable:
Red top
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| Collection Volume: |
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Preferred: Full 3.5 mL tube
Pediatrics: Pediatric tube filled to fill mark
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| Sample Analyzed: |
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Plasma or serum
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| Volume Required: |
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Preferred: 1 mL
Pediatrics: 0.5 mL
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| Specimen Processing: |
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Centrifuge specimen to yield platelet poor plasma/serum (platelet count should be less than 10 K/µL). Separate plasma/serum and transfer to plastic tube or vial. Freeze plasma/serum at -20°C or below.
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| Specimen Transport: |
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Transport whole blood specimen at room temperature to UWHC Core Laboratory (B4/220) within 4 hours of collection. See Specimen Processing for instructions if transport criteria are not met. Transport frozen aliquot on dry ice to UWHC Core Laboratory (B4/220).
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| Unacceptable Criteria: |
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Hemolyzed specimens, clotted specimens, partially filled tubes and whole blood specimens greater than 4 hours old when received by the laboratory are not acceptable.
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| Stability: |
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Ambient: 24 hours on a spun tube
Refrigerated: Not acceptable
Frozen: 2 weeks at -20°C; 6 months at -70°C
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Interpretation
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Expected Results:
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0 days and up: Negative
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| Additional Information: |
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The optical density (OD) of the reference calibrator and the patient are reported as an aid for interpretaton.
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HIT Antibodies Heparin Associated Platelet
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