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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Platelet Aggregation, Whole Blood

Test Code(s): WBPA / HCWBPA

CPT Code(s): 85576x4, 82397x5,85049,85014

Test Description: Test Component:

Includes Platelet count and Hematocrit


Methodology: Impedance/Luminescence

Clinical Significance: Test measures the ability of platelets to aggregate and/or release in whole blood in response to ADP, arachidonic acid, collagen, thrombin and ristocetin.

Days Performed: Mon-Fri, 0800-1300 - day varies. Specimens must be received in the laboratory by 1230. Testing required during off hours must be approved by a Coagulation physician. Call 263-5005 before collection.

Turnaround Time: Stat: 3 hours.
Routine: 3 hours.


Specimen Requirements

Specimen: Blood

Patient Preparation: The results may be affected if the patient has ingested aspirin or any anti-platelet medication,antihistamines,non-narcotic analgesics or NSAIDS prior to testing.

Collection Instructions: Do NOT draw through a line containing heparin. The venipuncture MUST be atraumatic to avoid hemolysis and contamination with tissue factors and platelet activators. Tube must be allowed to fill completely.

Please indicate patient history and if patient is receiving anticoagulant (Warfarin/Heparin/Vitamin K antagonist), direct thrombin inhibitor or fibrinolytic agent therapy and duration on request form.

Collection Container: Preferred: Light blue top (3.2% NaCitrate) and K3EDTA-Lavender top

Collection Volume: Preferred: 5 full 3.5 mL lt. blue tops and 1-4 mL lavender top
Pediatrics: Six full pediatric lt. blue tops filled to fill mark and 1-2 mL lavender top.

Sample Analyzed: Whole Blood

Specimen Processing: Do NOT freeze, refrigerate, or centrifuge. Do NOT open tube.

Specimen Transport: Hand deliver specimen to UWHC Core Laboratory (B4/220) ASAP. Do NOT send through a pneumatic tube. Specimens must be received by UWHC laboratory by 1230 Monday through Friday.

Unacceptable Criteria: Hemolyzed or clotted specimens are not acceptable. A partially filled tube is NOT acceptable. Patient ingestion of aspirin for 7 days and/or antihistamines, non-narcotic analgesics, or NSAIDs for 48 hours prior to sample collection is not acceptable.

Stability: Ambient: 3 hours unspun
Refrigerated: Not acceptable
Frozen: Not acceptable


Interpretation

Reference Interval:

Analyte Code

Analyte Description

UOM

Reference Range

Age/Gender

PLT

Platelet Count

K/µL

160-370

all ages

HCT

Hematocrit

%

44-70

0 days – 29 days

28-55

30 days-5 months

32-42

6 months-23 months

33-43

2-9 years

36-47

10-17 years,male

35-45

10-17 years, female

40-52

18 years and up, male

34-46

18 years and up, female

ADPA

ADP Aggregation

Ohms

7-36

all ages

ADPR

ADP Release

nmoles

0.3-1.9

all ages

ARACHA

Arachidonic Acid Aggregation

Ohms

8-36

all ages

ARACHR

Arachidonic Acid Release

nmoles

0.6-2.4

all ages

THROMR

Thrombin Release

nmoles

>0.5

all ages

LCOLLA

1 µg/mL Collagen Aggregation

Ohms

10-33

all ages

LCOLLR

1 µg/mL Collagen Release

nmoles

0.5-2.1

all ages

HCOLLA

5 µg/mL Collagen Aggregation

Ohms

15-35

all ages

HCOLLR

5 µg/mL Collagen Release

nmoles

0.9-2.6

all ages

LRISTO

0.15 mg/mL Ristocetin Aggregation

Ohms

<5

all ages

HRISTO

0.40 mg/mL Ristocetin Aggregation

Ohms

>5

all ages

NACLA

Spontaneous Aggregation

Ohms

<5

all ages

MCOAG1

Interpretation

 

 

 

 

 

 An interpretation by the Coagulation Lab Medical Director will be included in the report. A professional fee will be associated with the test.

 

Test Limitations:

Platelet count must be greater than 75 K/mcL for test results to be valid.

 

 

Additional Information:

NOTE: A Platelet Function Screening test utilizing the Dade Behring PFA-100 is also available. See "Platelet Function Screen" or call (608)263-5005 for information.

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