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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Culture, Blood, Bacteria and Yeast

Test Code(s): BLOC / GM4045 (for 1 collection site) or HCBLOC2 (if ordering 2 sites)

CPT Code(s): 87040; Additional CPT codes may be added for processing, identification, and susceptibility testing.

Methodology: Automated

Days Performed: Daily.

Turnaround Time: Stat: Not available stat.
Routine: Preliminary report: Negative cultures 7 - 24 hours. Final report: Negative cultures 5 days.


Specimen Requirements

Specimen: Blood

Collection Instructions: Please refer to "Blood Culture Volume Tables" for collection information: Go to "Specimen Collection and Container Types" listed under "More Information".

Collection Container: Preferred: Bactec® Blood Culture Bottle

Collection Volume: Preferred: 20 mL
Pediatrics: 4.5 mL

Sample Analyzed: Whole Blood

Specimen Transport: Transport specimen to UWHC Microbiology laboratory (B4/231).

Unacceptable Criteria: See Additional Information for rejection criteria.

Stability: Ambient: 2 days
Refrigerated: Not acceptable
Frozen: Not acceptable


Interpretation

Expected Results:

No growth

Critical Calls: View

Additional Information:

Best practices dictate that blood culture bottles should always be sent to the lab as soon as possible after blood collection for optimal results.

 

The method used detects aerobic and anaerobic bacteria as well as Candida and Cryptococcus. For adult patients, submit 15-20 mL from each of two separate sites. Culture of 30-40 mL per septic episode, not per fever spike, is sufficient to detect 99.9% of cases of sepsis in adults. For optimum diagnosis of sepsis, sample 3-4 sites only on the first day of a septic episode. Cultures on subsequent days are of minimal diagnostic value. If peripheral access is poor, one peripheral sample should be accompanied by a 15-20 mL line-drawn specimen.

 

INAPPROPRIATE SPECIMENS:

  • less than 5 mL in adult bottle
  • greater than 10 mL in adult bottle
  • only an anaerobic bottle received
  • pediatric bottle used on an adult patient
  • routine blood cultures accompanying approved quantitative blood cultures

 

Susceptibility testing is routinely performed except on organisms that are likely skin or air contaminants including single positive cultures growing coagulase-negative Staphylococcus.

 

PCR for MRSA and MSSA is routinely performed on positive blood cultures with gram-positive cocci in clusters. The assay cannot detect MSSA if MRSA is present.

 

Filamentous Fungus blood cultures should be drawn when Malassezia furfur or dimorphic fungi, e.g. Histoplasma capsulatum, are suspected or in the presence of negative routine blood cultures. See Culture, Blood, Filamentous Fungi entry.

 

For fastidious bacteria, contact the UWHC Director of Microbiology at (608) 263-4445 to discuss appropriate testing methods. If mycobacteria or cytomegalovirus is suspected, see specific entries. UWHC/UWMF providers please refer to the UConnect intranet site. See Best Practices for Blood Culturing.

Culture-Blood Routine
Blood Culture for Bacteria and Yeast (Routine)

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