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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Pneumocystis jirovecii Stain


CPT Code(s): 87015, 87207, 87205

Methodology: Microscopy

Days Performed: Mon-Fri, dayshift.

Turnaround Time: Stat: Not available stat.
Routine: 1 - 3 days.

Specimen Requirements

Specimen: See collection instructions.

Collection Instructions: Appropriate specimens include BAL, expectorated sputum, induced sputum. See additional information below.

HIV positive or suspected HIV positive patients: Collect either induced or expectorated sputa.

Bone marrow transplant, solid organ transplant and hematology patients: Collect induced sputa only.

Collection Container: Preferred: Sterile screw cap container

Specimen Transport: Transport specimen to UWHC Microbiology (B4/231) within 2 hours of collection.

Stability: Ambient: 2 hours
Refrigerated: 2 days
Frozen: Not acceptable


Expected Results:
 Pneumocystis jirovecii stain:  Negative
 Fungal elements stain:  Negative


Critical Calls: View

Additional Information:

Specimens must be received by 1100 for same day results of Pneumocystis stain.


Requests will be accepted on BAL specimens.


Requests will be accepted on either induced or expectorated sputa and BAL from HIV positive or suspected HIV positive patients. Requests from bone marrow transplant, solid organ transplant and hematology patients will be accepted on induced sputa and BAL only. Requests from any other patients must be reviewed by the Director of Microbiology or Pathology Resident.


Specimens must be acceptable using the same criteria as for sputum culture (< 10 squamous epithelial cells/ LPF).  On patients with HIV/ AIDS, the first specimen will be accepted regardless of the number of squamous epithelial cells seen on Gram stain.

TBO stain sputum

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