UW Hospital and Clinics Lab Test Directory
| Test Name: |
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Jo-1 Ab
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| Test Code(s): |
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JO1 / XJO1
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| CPT Code(s): |
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86235
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| Methodology: |
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Enzyme Immunoassay
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| Clinical Significance: |
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The Jo-1 antibodies test is an aid in the diagnosis of polymyositis and is not definitive in isolation. Antibodies to the Jo-1 antigen, also recognized as the enzyme histidyl-t RNA synthetase, are found in approximately 25% of patients with polymyositis. High proportions of patients with interstitial lung disease, associated with polymyositis, are positive for the antibody.
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| Days Performed: |
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Mon-Fri, dayshift - day varies.
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| Turnaround Time: |
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Stat: Not available stat.
Routine: 5 working days.
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Specimen Requirements
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| Specimen: |
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Blood
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| Collection Container: |
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Preferred: Red top
Also Acceptable:
Red cap with yellow ring (SST)
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| Collection Volume: |
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Preferred: 3 mL
Pediatrics: 1 mL
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| Sample Analyzed: |
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Serum
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| Volume Required: |
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Preferred: 1 mL
Pediatrics: 0.3 mL
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| Specimen Processing: |
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Centrifuge. Transfer cell-free serum to clear plastic vial. Refrigerate.
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| Specimen Transport: |
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Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from UWHC Satellite lab location.
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| Unacceptable Criteria: |
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Grossly hemolyzed, lipemic or turbid specimens should be avoided.
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| Stability: |
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Ambient: 8 hours
Refrigerated: 1 week
Frozen: At -20° C or colder, no limit. Note: Avoid repeat freeze and thaw.
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Interpretation
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Normal Jo-1 Ab (0 days and up): Negative
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Histidyl-t RNA synthetase antibodies, nti-Jo-1 antibodies
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