UW Hospital and Clinics Lab Test Directory
| Test Name: |
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Scl-70 Ab
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| Test Code(s): |
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SCL70 / XSCL70
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| CPT Code(s): |
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86235
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| Methodology: |
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Enzyme Immunoassay
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| Clinical Significance: |
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The Scl-70 Ab test is a diagnostic aid in the diagnosis of scleroderma and is not definitive in isolation. Antibodies to Scl-70 antigen, also recognized as the enzyme topoisomerase 1, are found in approximately 20% of scleroderma patients and are highly diagnostic of the disease. In the diffuse form of scleroderma, the frequency of antibodies to Scl-70 may be as high as 70%.
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| Days Performed: |
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Mon-Fri, dayshift - day varies.
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| Turnaround Time: |
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Stat: Not available stat.
Routine: 5 working days.
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Specimen Requirements
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| Specimen: |
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Blood
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| Collection Container: |
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Preferred: Red top
Also Acceptable:
Red cap with yellow ring (SST)
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| Collection Volume: |
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Preferred: 3 mL
Pediatrics: 1 mL
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| Sample Analyzed: |
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Serum
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| Volume Required: |
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Preferred: 1 mL
Pediatrics: 0.3 mL
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| Specimen Processing: |
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Centrifuge. Transfer cell-free serum to clear plastic vial. Refrigerate.
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| Specimen Transport: |
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Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from UWHC Satellite lab location.
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| Unacceptable Criteria: |
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Grossly hemolyzed, lipemic and turbid specimens are not acceptable.
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| Stability: |
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Ambient: 8 hours
Refrigerated: 1 week
Frozen: No limit at -20° C or lower. Note: Avoid repeat freeze and thaw.
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Interpretation
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Normal Scl-70 Ab (0 days and up): Negative
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Topoisomerase 1 antibodies Scl-70 Anti topoismerase 1
Anti-Scl-70 Antibodies,Anti-poisomerase Antibody,Anti-topirimase,Scleroderma Antibody
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