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American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: DNA Ab, double-stranded

Test Code(s): ADNA / DNA

CPT Code(s): 86225

Methodology: Enzyme Immunoassay

Clinical Significance: DNA antibodies, double-stranded (dsDNA) testing is performed in the evaluation of the patient suspected to have SLE.

dsDNA antibodies are found in 60% of patients with SLE, most having active disease. Other connective tissue diseases and drug-induced lupus rarely have dsDNA antibodies. Levels of dsDNA antibodies may rise and fall in association with disease activity in SLE (Ref: Arthritis Rheum 33:634-43,1990).

Days Performed: Thursdays.

Turnaround Time: Stat: Not available stat.
Routine: 5 working days.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red top
Also Acceptable: Red cap with yellow ring (SST)

Collection Volume: Preferred: 3 mL
Pediatrics: 1 mL

Sample Analyzed: Serum

Volume Required: Preferred: 1 mL
Pediatrics: 0.3 mL

Specimen Processing: Centrifuge. Transfer cell-free serum to clear plastic vial. Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from UWHC Satellite lab location or outreach location.

Unacceptable Criteria: Hemolyzed, lipemic and turbid specimens are not acceptable.

Stability: Ambient: Not acceptable
Refrigerated: 1 week
Frozen: 4 weeks at -20 C. Note: Avoid repeat freeze and thaw.


Expected Results:

0 days and up: Negative (0-50 IU/mL)


dsDNA Ab Interpretive Guidelines:


 0-50 IU/mL  Negative
 51-100 IU/mL  Low Positive
 101-200 IU/mL  Moderately Positive
 >200 IU/mL  High Positive



Additional Information:

An IFA procedure that uses the parasite Crithidia luciliae to confirm native DNA antibodies, double-stranded in patient sera is available through a reference laboratory upon receipt of request.

Anti-Double Stranded DNA Antibodies
Anti-dsDNA Antibodies
Anti-dsDNA Ab

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