UW Hospital and Clinics Lab Test Directory
| Test Name: |
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DNA Ab, double-stranded
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| Test Code(s): |
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ADNA / DNA
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| CPT Code(s): |
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86225
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| Methodology: |
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Enzyme Immunoassay
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| Clinical Significance: |
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DNA antibodies, double-stranded (dsDNA) testing is performed in the evaluation of the patient suspected to have SLE.
dsDNA antibodies are found in 60% of patients with SLE, most having active disease. Other connective tissue diseases and drug-induced lupus rarely have dsDNA antibodies. Levels of dsDNA antibodies may rise and fall in association with disease activity in SLE (Ref: Arthritis Rheum 33:634-43,1990).
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| Days Performed: |
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Mon-Fri, dayshift - day varies.
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| Turnaround Time: |
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Stat: Not available stat.
Routine: 5 working days.
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Specimen Requirements
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| Specimen: |
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Blood
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| Collection Container: |
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Preferred: Red top
Also Acceptable:
Red cap with yellow ring (SST)
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| Collection Volume: |
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Preferred: 3 mL
Pediatrics: 1 mL
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| Sample Analyzed: |
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Serum
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| Volume Required: |
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Preferred: 1 mL
Pediatrics: 0.3 mL
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| Specimen Processing: |
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Centrifuge. Transfer cell-free serum to clear plastic vial. Refrigerate.
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| Specimen Transport: |
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Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from UWHC Satellite lab location or outreach location.
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| Unacceptable Criteria: |
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Hemolyzed, lipemic and turbid specimens are not acceptable.
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| Stability: |
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Ambient: Not acceptable
Refrigerated: 1 week
Frozen: 4 weeks at -20° C. Note: Avoid repeat freeze and thaw.
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Interpretation
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Expected Results:
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0 days and up: Negative (0-50 IU/mL)
dsDNA Ab Interpretive Guidelines:
| 0-50 IU/mL |
Negative |
| 51-100 IU/mL |
Low Positive |
| 101-200 IU/mL |
Moderately Positive |
| >200 IU/mL |
High Positive |
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| Additional Information: |
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An IFA procedure that uses the parasite Crithidia luciliae to confirm native DNA antibodies, double-stranded in patient sera is available through a reference laboratory upon receipt of request.
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Anti-Double Stranded DNA Antibodies
Anti-dsDNA Antibodies
Anti-dsDNA Ab
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