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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Cardiolipin Ab, IgG/IgM

Test Code(s): ACARD / XCARGM

CPT Code(s): 86147x2

Test Description: Test Component:

IgG and IgM antibodies to cardiolipin

Methodology: Enzyme Immunoassay

Clinical Significance: Cardiolipin Ab testing is performed for evaluation of hypercoagulable state that may be associated with venous or arterial thromboemboli.

Diseases associated with cardiolipin antibodies include SLE, drug-induced lupus, other connective tissue diseases and primary anti-phospholipid antibody syndrome.

Days Performed: Mon-Fri, dayshift - day varies.

Turnaround Time: Stat: Not available stat.
Routine: 5 working days.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red top
Also Acceptable: Red cap with yellow ring (SST)

Collection Volume: Preferred: 3 mL
Pediatrics: 1 mL

Sample Analyzed: Serum

Volume Required: Preferred: 1 mL
Pediatrics: 0.3 mL

Specimen Processing: Centrifuge. Transfer cell-free serum to plastic vial.  Refrigerate.

Specimen Transport: Transport serum sample to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from UWHC Satellite lab location.

Outreach Specimen Transport: Transport serum sample to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Unacceptable Criteria: Grossly hemolyzed, lipemic and turbid specimens are not acceptable.

Stability: Ambient: 8 hours
Refrigerated: 7 days
Frozen: No limit at -20 C. Note: Avoid repeat freeze and thaw.


Expected Results:

0 days and up:

IgG:  Negative (0-15 GPL)
IgM:  Negative (0-13 MPL)


Cardiolipin Ab Interpretive Guidelines:

0-15:  Negative 
16-20:  Indeterminate
21-80:  Low-Medium Positive
>80:  Positive 


0-13:  Negative
14-20:  Indeterminate
21-80:  Low-Medium Positive
>80:  Positive

Cardiolipin Ab results are obtained with the INOVA QUANTA Lite ACA IgG and IgM (HRP)ELISA kits.



Test Limitations:

Rheumatoid factor, usually an IgM autoantibody, can interfere with the determination of the IgM aCL. Cardiolipin Ab, IgG and IgM values obtained with different manufacturers' assay methods may not be used interchangeably. The magnitude of the reported IgG or IgM levels cannot be correlated to an endpoint titer.

Anti Cardiolipin IgM
Antibody IgG
Anti-Cardiolipin Antibodies Panel

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