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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Immunofixation Electrophoresis

Test Code(s): IFIX / GM3070

CPT Code(s): 86334

Methodology: Gel Electrophoresis

Clinical Significance: Immunofixation is used to detect and to type monoclonal gammopathies (IgA, IgG, IgM, kappa or lambda) in cases where restricted heterogeneity is found on protein electrophoresis.

Days Performed: Mon-Fri, dayshift.

Turnaround Time: Stat: Not available stat.
Routine: 3 working days.


Specimen Requirements

Specimen: Blood

Collection Instructions: If there is the possibility that the patient has a cryoprotein, specimen MUST BE DRAWN AND KEPT WARM UNTIL AFTER TESTING IS COMPLETE.

BEFORE COLLECTING SPECIMEN, pre-warm a collection tube (e.g. in an infant heel warmer). Have another infant heel warmer on hand to deliver the blood to the laboratory after collection. Keep specimen at 37C by wrapping a hot pack (infant heel warmer) around the tube immediately after collection. Transport specimen to Core Laboratory (B4/220) within 10 minutes of collection. Contact laboratory if unable to assure delivery within 10 minutes.

Collection Container: Preferred: Red top
Also Acceptable: Red cap with yellow ring (SST)

Collection Volume: Preferred: 3 mL
Pediatrics: 1 mL

Sample Analyzed: Serum

Volume Required: Preferred: 1 mL
Pediatrics: 0.2 mL

Specimen Processing: Centrifuge and transfer cell-free serum to plastic vial. Refrigerate.

IF THE PATIENT HAS A CRYOPROTEIN, keep blood specimen at 37 C during entire processing procedure. Place collection tube in the 37C incubator for two hours. Spin tube at 3000 rpm for 10 minutes at 37C. Remove spun serum to labeled tube. Place WARM sticker on tube and refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from UWHC Satellite Lab locations.

IF THE PATIENT HAS A CRYOPROTEIN, transport blood specimen to UWHC Core Laboratory (B4/220) within ten minutes of collection. Keep specimen at 37C by wrapping tube in a hot pack (infant heel warmer). Specimen MUST be processed using the Specimen Processing procedure above prior to transport if coming from UWHC Satellite lab locations. Write WARM on the tube and transport specimen with coolant pack after processing is complete.

Outreach Specimen Transport: IF THE PATIENT HAS A CRYOPROTEIN, specimen MUST be collected and processed using the Collection and Specimen Processing procedures above prior to transport. Write WARM on the tube and transport specimen with coolant pack after processing is complete.

Unacceptable Criteria: Grossly hemolyzed specimens are not acceptable.

Stability: Ambient: Not acceptable
Refrigerated: 1 week
Frozen: 3 months at -20 C. Note: Avoid repeat freeze and thaw.


Interpretation

A written interpretive report is issued as a separate report.

Additional Information:

A professional fee is associated with this test.

Immunoelectrophoresis  IFIX  serum protein immunofixation serum immunofixation  serum IFIX serum IEP

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