| Test Name: |
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IgA
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| Test Code(s): |
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IGA / XIGA
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| CPT Code(s): |
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82784
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| Methodology: |
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Nephelometry
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| Clinical Significance: |
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Measurement of IgA aids in the diagnosis of humoral immunodeficiencies, ataxia-telangiectasia, multiple myeloma and hypergammaglobulinemic states.
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| Days Performed: |
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Daily, 24 hours.
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| Turnaround Time: |
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Stat: 1 hour.
Routine: Up to 4 hours.
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Specimen Requirements
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| Specimen: |
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Blood
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| Collection Instructions: |
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If there is the possibility that the patient has a cryoprotein, specimen MUST BE KEPT WARM UNTIL AFTER TESTING IS COMPLETE. Indicate specimen is to be drawn warm.
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| Collection Container: |
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Preferred: Red cap with yellow ring (SST)
Also Acceptable:
Red top
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| Collection Volume: |
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Preferred: 3 mL
Pediatrics: 1 mL
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| Sample Analyzed: |
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Serum
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| Volume Required: |
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Preferred: 1 mL
Pediatrics: 0.3 mL
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| Specimen Processing: |
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Centrifuge within 2 hours of draw. Transfer cell-free serum to plastic vial. Refrigerate. Freeze serum if not assayed within 72 hours.
IMPORTANT:
IF THE PATIENT HAS A CRYOPROTEIN, keep blood specimen at 37°C during entire processing procedure. Place collection tube in the 37°C incubator for two hours. Spin tube at 3000 rpm for 10 minutes at 37°C. Remove spun serum to labeled tube and forward to Chemistry Lab.
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| Specimen Transport: |
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Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location. Transport frozen serum aliquot on dry ice to UWHC Core Laboratory (B4/220).
Important:
For samples on patients suspected of having a cryoprotein, specimen MUST be processed prior to transport if coming from outreach location. Transport specimens with coolant pack after processing is complete.
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| Unacceptable Criteria: |
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Grossly hemolyzed specimens are not acceptable.
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| Stability: |
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Refrigerated: 7 days
Frozen: 3 months at -20° C or below. Note: Avoid repeat freeze and thaw.
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Interpretation
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Reference Interval:
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| Age: |
mg/dL |
| 0 days-29 days |
7-94 |
| 30 days-11 months |
10-131 |
| 12 months-23 months |
19-175 |
| 24 months-35 months |
22-220 |
| 3 years-4 years |
48-345 |
| 5 years-9 years |
41-297 |
| 10 years-12 years |
44-395 |
| 13 years-17 years |
44-441 |
| 18 years and up |
50-450 |
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