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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: HLA-B27

Test Code(s): B27 / XHLA

CPT Code(s): 81374

Methodology: Reverse SSO detected by Luminex xMAP (Multi-Analyte Profiling)

Clinical Significance: The HLA-B27 antigen is associated with Ankylosing Spondylitis and several other related diseases.  The absence of B27 may be used to rule out the associated disease.

Days Performed: Mon-Fri.

Turnaround Time: Stat: Not available stat.
Routine: 2 days.

Specimen Requirements

Specimen: Blood

Collection Instructions: Do NOT open tube.

Collection Container: Preferred: Lavender top
Also Acceptable: Yellow top(with ACD-A/B anticoagulant)

Collection Volume: Preferred: Full 4 mL tube
Pediatrics: 2 mL

Volume Required: Preferred: 4 mL
Pediatrics: 2 mL

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220) immediately.

Stability: Ambient: 7 days
Refrigerated: 7 days
Frozen: Not acceptable



 Negative:  HLA-B27 antigen not detected
 Positive:  HLA-B27 antigen detected



Additional Information:

This test was developed and its performance characteristics determined by this laboratory.  It has not been cleared or approved by the US Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA-88) as qualified to perform high complexity clinical laboratory testing.

ankylosing spondylitis

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