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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: PSA, Total, Diagnostic

Test Code(s): PSAD / PSA

CPT Code(s): 84153

Methodology: Chemiluminescent Immunoassay

Days Performed: Daily, 24 hours.

Turnaround Time: Stat: 1 hour.
Routine: 4 hours.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red cap with yellow ring (SST)
Also Acceptable: Red top

Collection Volume: Preferred: 2 mL
Pediatrics: 0.3 mL

Sample Analyzed: Serum

Volume Required: Preferred: 1 mL
Pediatrics: 0.15 mL

Specimen Processing: Centrifuge within 2 hours of collection.  If a complete barrier has not formed, transfer cell-free serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Stability: Refrigerated: 6 days
Frozen: 4 months


Reference Interval:

18 years and up:  0.00-4.00 ng/mL

Test Limitations:

Testing performed on Siemen's Diemension Vista.

Samples should be obtained before biopsy, prostatectomy or prostatic massage, since manipulation of the prostate gland may lead to elevated PSA levels persisting for up to 3 weeks.

Studies have shown conflicting results on the existence of an effect of digital rectal examination on PSA level. Therefore, when possible, obtain PSA samples before digital rectal examination.

PSA expression may be altered due to hormonal therapy for prostate cancer. Consequently, a low PSA result following a prostatic cancer treatment which includes hormonal therapy may not adequately reflect the presence of residual or recurrent disease.

Results may be affected if patient has developed Human Anti-Murine Antibodies (HAMA) due to exposure to murine based products

Heterophilic antibodies in human serum can react with the immunoglobulins included in the assay components causing interference with in vitro immunoassays. Samples from patients routinely exposed to animals or animal serum products can demonstrate this type of interference potentially causing an anomalous result.

A high dose hook effect is not seen up to 50,000 ng/mL.

Additional Information:

The PSA assay may be used in conjunction with a digital rectal exam as a screening test for prostate cancer.

Prostate Specific Antigen- Total, Diagnostic

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