| Test Name: |
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Vancomycin
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| Test Code(s): |
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VANC / HCVANC
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| CPT Code(s): |
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80202
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| Methodology: |
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Particle Enhanced Turbidometric Inhibition Immunoassay (PETINIA)
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| Clinical Significance: |
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Antibiotic
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| Days Performed: |
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Daily, 24 hours.
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| Turnaround Time: |
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Stat: 1 hour.
Routine: 4 hours.
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Specimen Requirements
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| Specimen: |
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Blood
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| Collection Instructions: |
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Collect specimen for peak level 2 hours after the end of a 60 minute infusion. Collect trough specimen 15 - 30 minutes prior to next dose.
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| Collection Container: |
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Preferred: Red top
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| Collection Volume: |
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Preferred: 2 mL
Pediatrics: 0.4 mL
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| Sample Analyzed: |
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Serum
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| Volume Required: |
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Preferred: 1 mL
Pediatrics: 0.2 mL
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| Specimen Processing: |
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Centrifuge. Transfer cell-free serum to plastic vial. Refrigerate.
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| Specimen Transport: |
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Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.
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| Unacceptable Criteria: |
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Specimens collected in red cap with yellow ring (SST) tubes are not acceptable.
Specimens greater than 2 hours old, uncentrifuged when received by the laboratory, are not acceptable.
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| Stability: |
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Ambient: 2 hours on cells; 8 hours off cells
Refrigerated: 3 days
Frozen: 1 month
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Interpretation
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Optimal therapeutic range: Trough 5.0 - 10.0 mcg/mL
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| Test Limitations: |
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Penicillin G at concentrations of 25 U/ml [25000 U/L] increases vancomycin results by 13% at a vancomycin concentration of 24.0 mcg/mL.
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| Critical Calls: |
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View
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| Additional Information: |
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Result of "BELOW" indicates that the result is below the method detection limit of 0.8 mcg/mL. Vancomycin is a glycopeptide antibiotic which is bactericidal against many gram-positive and some gram-negative cocci. It is useful in therapy of infection in patients who cannot receive or who have failed to respond to the penicillins and cephalosporins.
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