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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Vancomycin

Test Code(s): VANC / HCVANC

CPT Code(s): 80202

Methodology: Particle Enhanced Turbidometric Inhibition Immunoassay (PETINIA)

Clinical Significance: Antibiotic

Days Performed: Daily, 24 hours.

Turnaround Time: Stat: 1 hour.
Routine: 4 hours.

Specimen Requirements

Specimen: Blood

Collection Instructions: Collect specimen for peak level 2 hours after the end of a 60 minute infusion. Collect trough specimen 15 - 30 minutes prior to next dose.

Collection Container: Preferred: Red top

Collection Volume: Preferred: 2 mL
Pediatrics: 0.4 mL

Sample Analyzed: Serum

Volume Required: Preferred: 1 mL
Pediatrics: 0.2 mL

Specimen Processing: Centrifuge.  Transfer cell-free serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Unacceptable Criteria: Specimens collected in red cap with yellow ring (SST) tubes are not acceptable.
Specimens greater than 2 hours old, uncentrifuged when received by the laboratory, are not acceptable.

Stability: Ambient: 2 hours on cells; 8 hours off cells
Refrigerated: 3 days
Frozen: 1 month


Optimal therapeutic range: Trough 5.0 - 10.0 mcg/mL

Test Limitations:

Penicillin G at concentrations of 25 U/ml [25000 U/L] increases vancomycin results by 13% at a vancomycin concentration of 24.0 mcg/mL.

Critical Calls: View

Additional Information:

Result of "BELOW" indicates that the result is below the method detection limit of 0.8 mcg/mL. Vancomycin is a glycopeptide antibiotic which is bactericidal against many gram-positive and some gram-negative cocci. It is useful in therapy of infection in patients who cannot receive or who have failed to respond to the penicillins and cephalosporins.

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