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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Prolactin

Test Code(s): PRLT / PROL

CPT Code(s): 84146

Methodology: Chemiluminescent Immunoassay

Days Performed: Mon-Fri, dayshift.

Turnaround Time: Routine: Next working day.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red cap with yellow ring (SST)
Also Acceptable: Red top

Collection Volume: Preferred: 1 mL
Pediatrics: 1 mL

Sample Analyzed: Serum

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.5 mL

Specimen Processing: Centrifuge.  If a complete barrier has not formed, transfer cell-free serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Stability: Ambient: 8 hours
Refrigerated: 48 hours
Frozen: Freeze if sample will not be assayed within 48 hours.


Reference Interval:




 Age  ng/mL
 1-9 years  
       Male  0.2 - 13.0
       Female  0.2 - 13.0
10 years and up  
       Male  0.2 - 13.0
       Female   0.2 - 24.5


Test Limitations:

Results may be affected if patient has developed heterophilic antibodies due to exposure to animal based protein products.

Additional Information:

A high dose hook effect is not seen up to 15,000 ng/mL.

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