| Test Name: |
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Differential without WBC
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| Test Code(s): |
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D1 / DIFF
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| CPT Code(s): |
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85004
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| Test Description: |
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Test Component: WBC Differential, RBC morphology and platelet evaluation.
Use of manual microscopy for WBC differential is determined by laboratory personnel during testing.
Included In: CBC with Differential, WBC and Differential
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| Methodology: |
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Flow Cytometry, Impedance, and where applicable, Manual Microscopy
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| Days Performed: |
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Daily, 24 hours- all ER specimens performed stat and General Peds/Adolescent Med (GPAM) specimens performed stat upon request (please mark on request form).
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| Turnaround Time: |
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Stat: 1 hour.
Routine: 4 hours.
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Specimen Requirements
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| Specimen: |
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Blood
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| Collection Container: |
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Preferred: K3EDTA-Lavender top
Also Acceptable:
K2EDTA-Lavender top
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| Collection Volume: |
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Preferred: 4 mL
Pediatrics: 1 mL
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| Sample Analyzed: |
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Whole Blood
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| Volume Required: |
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Preferred: 1 mL
Pediatrics: 0.25 mL in microtainer
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| Specimen Transport: |
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Transport specimen to laboratory at ambient temperature.
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| Unacceptable Criteria: |
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Clotted specimens are not acceptable. Tubes with <25% of fill volume.
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| Stability: |
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Ambient: 8 hours
Refrigerated: Not acceptable
Frozen: Not acceptable
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Interpretation
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Reference Interval:
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Note: Reference Intervals for relative percent (%) can be found on the patient report.
| Age 18 years and up: |
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| WBC |
Neuts |
Lymphs |
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| K/uL |
cells/uL |
cells/uL |
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| 3.8-10.5 |
1700-7500 |
1000-3500 |
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| Monos |
Eos |
Basos |
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| cells/uL |
cells/uL |
cells/uL |
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| 200-900 |
0-500 |
0-200 |
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| Pediatric Reference Interval* |
| Age |
WBC |
Neutrophils |
Lymphocytes |
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K/uL |
cells/uL |
cells/uL |
| 0-29 days |
9.1-34.0 |
6000-23500 |
2500-10500 |
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30 days-23
months
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6.0-14.0 |
1100-6600 |
1800-9000 |
| 2-9 years |
4.0-12.0 |
1400-6600 |
1000-5500 |
| 10-17 years |
4.0-10.5 |
1500-6600 |
1000-3500 |
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| Age |
Monocytes |
Eosinophils |
Basophils |
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cells/uL |
cells/uL |
cells/uL |
| 0-29 days |
< 3500 |
< 2000 |
< 400 |
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30 days-23
months
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< 1000 |
< 700 |
< 100 |
| 2-9 years |
< 1000 |
< 700 |
< 100 |
| 10-17 years |
< 1000 |
< 700 |
< 100 |
*from Children's Hospital of Buffalo, 1995/Verified by
Milwaukee Children's Hospital
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| Critical Calls: |
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View
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