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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: CK, Total and CK-MB with Index, if indicated

Test Code(s): CPKMBI

CPT Code(s): 82550, 82553

Test Description: Test Component:

CK,Total, CK-MB isoenzyme, and calculated CK-Index

Methodology: See individual tests.

Days Performed: Daily, 24 hours.

Turnaround Time: Stat: 1 hour.
Routine: 4 hours.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Green cap with yellow ring (PST)
Also Acceptable: Green top, Red cap with yellow ring (SST), Red top

Collection Volume: Preferred: 1 mL
Pediatrics: 0.5 mL

Sample Analyzed: Plasma or serum

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.3 mL

Specimen Processing: Centrifuge. If a complete barrier has not formed, transfer cell-free plasma/serum to clear plastic vial. Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Stability: Ambient: 12 hours
Refrigerated: 2 days
Frozen: 1 month



CK, Total Reference Interval:  
Age 12 months and up:  
   Males:  0-250 U/L
   Females:  0-175 U/L


Interpretive Guidelines for CKMB and CK-Index:



 CKMB (ng/mL)


 Non-Acute Myocardial Infarction



 Gray Zone



 Acute Myocardial Infarction




Peak values of CK-MB greater than 5.0 ng/mL AND CK-Index greater than 4.0 are highly indicative of myocardial damage. Diagnosing myocardial injury with the aid of laboratory medicine is best done using serial samples collected over the 24-36 hours following the suspected event.

Creatine Kinase-Index
CK Index

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