UW Hospital and Clinics Lab Test Directory
| Test Name: |
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Mono Test
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| Test Code(s): |
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MONO / MSP
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| CPT Code(s): |
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86308
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| Methodology: |
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Immunochromatographic Assay
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| Days Performed: |
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Daily, 24 hours.
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| Turnaround Time: |
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Stat: 1 hour.
Routine: 4 hours.
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Specimen Requirements
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| Specimen: |
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Blood
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| Collection Container: |
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Preferred: Red top
Also Acceptable:
Lavender top,
Red cap with yellow ring (SST)
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| Collection Volume: |
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Preferred: 1 mL
Pediatrics: 0.5 mL
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| Sample Analyzed: |
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Serum or plasma
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| Volume Required: |
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Preferred: 0.5 mL
Pediatrics: 0.25 mL
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| Specimen Processing: |
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Centrifuge. Transfer cell-free serum/plasma to plastic vial. Refrigerate.
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| Specimen Transport: |
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Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.
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| Unacceptable Criteria: |
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Hemolyzed specimens are not acceptable.
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| Stability: |
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Refrigerated: 2 days
Frozen: 1 year; avoid repeat freeze/thaw cycles
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Interpretation
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Expected Results:
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Negative
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| Test Limitations: |
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NEGATIVE: Heterophile antibodies are usually detectable within 3 wk of EBV infection. During the acute phase of illness, they are detectable in 80-85% of IM cases, about 50% in children under 4 years of age. If clinical suspicion persists, testing for IgM to EBV VCA may be warranted.
POSITIVE: A positive test provides support for the diagnosis of IM due to EBV. Note that heterphile antibodies may persist for months or, in 2-3% of patients, more than 1 year following acute EBV infection. The presence of EBV VCA IgM would be confirmatory of current disease.
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Mono Spot
infectious mononucleosis
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