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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Mono Test

Test Code(s): MONO / MSP

CPT Code(s): 86308

Methodology: Immunochromatographic Assay

Days Performed: Daily, 24 hours.

Turnaround Time: Stat: 1 hour.
Routine: 4 hours.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red top
Also Acceptable: Lavender top, Red cap with yellow ring (SST)

Collection Volume: Preferred: 1 mL
Pediatrics: 0.5 mL

Sample Analyzed: Serum or plasma

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.25 mL

Specimen Processing: Centrifuge.  Transfer cell-free serum/plasma to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Unacceptable Criteria: Hemolyzed specimens are not acceptable.

Stability: Refrigerated: 2 days
Frozen: 1 year; avoid repeat freeze/thaw cycles


Expected Results:


Test Limitations:

NEGATIVE:  Heterophile antibodies are usually detectable within 3 wk of EBV infection. During the acute phase of illness, they are detectable in 80-85% of IM cases, about 50% in children under 4 years of age. If clinical suspicion persists, testing for IgM to EBV VCA may be warranted.
POSITIVE:  A positive test provides support for the diagnosis of IM due to EBV. Note that heterphile antibodies may persist for months or, in 2-3% of patients, more than 1 year following acute EBV infection. The presence of EBV VCA IgM would be confirmatory of current disease.

Mono Spot
infectious mononucleosis

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