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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Methemoglobin

Test Code(s): MHGB / XMHGB

CPT Code(s): 83050

Methodology: Spectrophotometry

Days Performed: Daily, 24 hours.

Turnaround Time: Stat: 1 hour. All requests processed stat.

Specimen Requirements

Specimen: Arterial, venous, or capillary

Collection Instructions: Specimen must be free of air bubbles and clots.
For capillary specimens, collect two full Radiometer capillaries.

Collection Container: Preferred: Radiometer PICOŽ syringe
Also Acceptable: 2 full Radiometer capillary tubes

Collection Volume: Preferred: 1.5 mL
Pediatrics: 0.5 mL

Sample Analyzed: Whole Blood

Volume Required: Preferred: 1.5 mL
Pediatrics: 0.5 mL

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220)immediately.  Specimen must arrive in the UWHC Core Lab within 30 minutes of collection.

Unacceptable Criteria: Clotted specimens, specimens with air in the syringe, or specimens past stability time are not acceptable.

Stability: Ambient: 30 minutes
Refrigerated: 1 hour
Frozen: Not acceptable


Expected Results:

Up to 1.5% of total hemoglobin

Test Limitations:

Interferences due to lipemia or sulfhemoglobinemia are rare but may occur.

Critical Calls: View

Additional Information:

Cyanosis is evident when level reaches 10 - 15% of total hemoglobin.

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