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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: LH

Test Code(s): LH

CPT Code(s): 83002

Methodology: Chemiluminescent Immunoassay

Days Performed: Mon-Fri, dayshift.

Turnaround Time: Stat: Not available stat.
Routine: Next working day.


Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red cap with yellow ring (SST)
Also Acceptable: Red top

Collection Volume: Preferred: 1 mL
Pediatrics: 0.5 mL

Sample Analyzed: Serum

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.15 mL

Specimen Processing: Centrifuge.  If a complete barrier has not formed, transfer cell-free serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Stability: Ambient: 8 hours
Refrigerated: 48 hours
Frozen: Freeze if sample will not be assayed within 48 hours.


Interpretation

Reference Interval:
 Male:  mIU/mL
   16 days - 10 years  0.3 - 2.8
   11 years  0.3 - 1.8
   12 years  0.3 - 4.0
   13 years  0.3 - 6.0
   14 years  0.5 - 7.9
   15 years - 16 years  0.5 - 10.8
   17 years  0.9 - 5.9
   18 years - 70 years  1.5 - 9.3
   71 years and up  3.1 - 34.0
   
 Female:  
   16 days - 10 years  0.2 - 3.0
   11 years  0.2 - 6.5
   12 years  0.4 - 9.9
   13 years  0.3 - 5.4
   14 years  0.5 - 31.2
   15 years  0.5 - 20.7
   16 years  0.4 - 29.4
   17 years  1.6 - 12.4
    
 Female 18 years and up:  mIU/mL
   Follicular phase   1.9 - 12.5
   Midcycle peak   8.7 - 76.3
   Luteal phase   0.5 - 16.9
   Pregnant  < 0.1 - 1.5
   Postmenopausal   15.9 - 54.0
   Contraceptives   0.7 - 5.6

 

Pediatric reference intervals from Reference Ranges for Serum Concentrations of Lutropin (LH), Follitropin (FSH), Estradial (E2), Prolactin, Progesterone, Sex Hormone-Binding Globulin (SHBG), Dehydroepiandrosterone Sulfate (DHEAS), Cortisol and Ferritin in Neonates, Children and Young Adults, Clin Chem Lab Med 2002; 40(11):1151-1160.

Test Limitations:

Results may be affected if patient has developed heterophilic antibodies due to exposure to animal based protein products.

Additional Information:

A high dose hook effect is not seen up to 18,000 mIU/mL.

Luteinizing Hormone

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