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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: LD, Total

Test Code(s): LD / LDH

CPT Code(s): 83615

Methodology: Lactate-Pyruvate

Days Performed: Daily, 24 hours.

Turnaround Time: Stat: 1 hour.
Routine: 4 hours.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Green cap with yellow ring (PST)
Also Acceptable: Green top, Red cap with yellow ring (SST), Red top

Collection Volume: Preferred: 1 mL
Pediatrics: 0.4 mL

Sample Analyzed: Plasma or serum

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.2 mL

Specimen Processing: Centrifuge.  Redraw specimen if hemolysis is present.  If a complete barrier has not formed, transfer cell-free plasma/serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Unacceptable Criteria: Hemolyzed specimens or whole blood specimens greater than 2 hours old when received by the laboratory are not acceptable.

Stability: Ambient: 24 hours
Refrigerated: PST - 48 hrs; if off cells or SST - 4 days
Frozen: Not acceptable


Reference Interval:
 Age  U/L
  0 days - 30 days  180-730
 31 days - 11 months  200-580
 1-3 years  225-430
 4-6 years  200-400
 7-9 years  200-370
 10-13 years  150-340
 14-16 years  150-310
 17 years and up  90-245


LDH Lactate Dehydrogenase

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