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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Iron/ TIBC with Saturation

Test Code(s): IRON / FEPSAT

CPT Code(s): 83540, 83550

Test Description: Test Component:

Iron, total iron binding capacity and saturation.

Methodology: Ferene

Request Form: UWHC Laboratory Request Form (UWH 1289220, 4004202); Write in test if not on form.

Days Performed: Daily, 24 hours.

Turnaround Time: Stat: 1 hour.
Routine: 4 hours.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Green cap with yellow ring (PST)
Also Acceptable: Green top, Red cap with yellow ring (SST), Red top

Collection Volume: Preferred: 1 mL
Pediatrics: 0.5 mL

Sample Analyzed: Serum or plasma

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.2 mL

Specimen Processing: Centrifuge.  If a complete barrier has not formed, transfer cell-free serum/plasma to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Unacceptable Criteria: Grossly hemolyzed specimens are not acceptable.

Stability: Refrigerated: 7 days
Frozen: 6 months- must be stored in iron-free container


Reference Interval:
    Age  mcg/dL
    0-30 days  100-250
    31 days - 2 years  40-100
    3-17 years  50-120
    18 years and up  50-160
    0 days - 2 years  100-400
    3 years and up  250-450
    3 years and up:  16-50%

Additional Information:

Measurements of Iron and Total Iron Binding Capacity may be inaccurate if performed within 14 days of IV iron dextran administration.

Transferrin Saturation  TIBC

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