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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: HIV-1, 2 Ab

Test Code(s): HIVAB / HIV

CPT Code(s): 87806

Methodology: Chemiluminescent Immunoassay

Clinical Significance: Detects antibodies to HIV-1, including group 0, and/or HIV-2

Days Performed: Mon-Fri, dayshift.

Turnaround Time: Stat: 2 hours.
Routine: Next working day.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red top
Also Acceptable: Red cap with yellow ring (SST)

Collection Volume: Preferred: 4 mL
Pediatrics: 3 mL

Sample Analyzed: Serum

Volume Required: Preferred: 2 mL
Pediatrics: 1.5 mL

Specimen Processing: Centrifuge.  Transfer cell-free serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Stability: Ambient: 24 hours
Refrigerated: Primary tube: 5 days; Secondary tube: 7 days
Frozen: okay; no repeat freeze/thaw cycles


Expected Results:


Test Limitations:

The performance of this assay has not been established with cord blood, neonatal specimens, heat inactivated specimens or body fluids other than serum.

It is recognized that currently available assays for the detection of antibodies to HIV-1 and/or HIV-2 may not detect all infected individuals. A negative test result does not exclude the possibility of exposure to or infection with HIV. HIV antibodies may be undetectable in some stages of the infection and in some clinical conditions.

Additional Information:

Reactive specimens are sent to a reference lab for confirmation.


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