| Test Name: |
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HIV-1, 2 Ab
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| Test Code(s): |
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HIVAB / HIV
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| CPT Code(s): |
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86703
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| Methodology: |
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Chemiluminescent Immunoassay
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| Clinical Significance: |
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Detects antibodies to HIV-1, including group 0, and/or HIV-2
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| Days Performed: |
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Mon-Fri, dayshift.
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| Turnaround Time: |
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Stat: 2 hours.
Routine: Next working day.
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Specimen Requirements
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| Specimen: |
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Blood
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| Collection Container: |
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Preferred: Red top
Also Acceptable:
Red cap with yellow ring (SST)
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| Collection Volume: |
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Preferred: 4 mL
Pediatrics: 3 mL
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| Sample Analyzed: |
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Serum
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| Volume Required: |
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Preferred: 2 mL
Pediatrics: 1.5 mL
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| Specimen Processing: |
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Centrifuge. Transfer cell-free serum to plastic vial. Refrigerate.
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| Specimen Transport: |
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Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.
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| Stability: |
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Ambient: 24 hours
Refrigerated: Primary tube: 5 days; Secondary tube: 7 days
Frozen: okay; no repeat freeze/thaw cycles
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Interpretation
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Expected Results:
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Nonreactive
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| Test Limitations: |
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The performance of this assay has not been established with cord blood, neonatal specimens, heat inactivated specimens or body fluids other than serum.
It is recognized that currently available assays for the detection of antibodies to HIV-1 and/or HIV-2 may not detect all infected individuals. A negative test result does not exclude the possibility of exposure to or infection with HIV. HIV antibodies may be undetectable in some stages of the infection and in some clinical conditions.
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| Additional Information: |
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Reactive specimens are sent to a reference lab for confirmation.
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