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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Hepatitis C Ab

Test Code(s): HEPC / HCV

CPT Code(s): 86803

Methodology: Chemiluminescent Immunoassay

Clinical Significance: Detects IgG antibody to Hepatitis C virus.

Days Performed: Mon-Fri, dayshift.

Turnaround Time: Stat: Not available stat.
Routine: Next working day.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red cap with yellow ring (SST)
Also Acceptable: Red top

Collection Volume: Preferred: 1.5 mL
Pediatrics: 0.5 mL

Sample Analyzed: Serum

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.1 mL

Specimen Processing: Centrifuge.  If complete barrier has not formed, transfer cell-free serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Stability: Refrigerated: 7 days
Frozen: >7 days



Reference Interval:  Non-reactive


Interpretive Guidelines:


Non-reactive:  The method detects IgG antibodies to Hepatitis C virus. A negative test result does not exclude the possibility of exposure to HCV. There may be a delay in the production of antibodies to the virus. You may repeat the test in four to eight weeks to determine if antibodies have been produced.


Equivocal:  CDC recommends confirmatory testing of equivocal results.  Please place a new order for Hepatitis C RNA, Quant, PCR [XHCVRQ]


Reactive - Index value :

CDC recommends confirmatory testing of reactive results.  Please place a new order for Hepatitis C RNA, Quant, PCR [XHCVRQ] 


Reactive - Index value ≥11:

95% of the time this represents past or present infection with HCV. Supplemental testing was not performed. You may order Hepatitis C RNA, Quant, PCR [XHCVRQ] testing if clinically indicated.

Test Limitations:

Results may be affected if patient has developed heterophilic antibodies due to exposure to animal based protein products.


The assay is an in vitro diagnostic immunoassay for qualitative determination of immunoglobulin G (IgG) antibodies to hepatitis C virus.


The assay performance characteristics have not been established for immunocompromised or immunosuppressed patients, newborns, infants or children, cord blood, neonatal specimens, cadaver specimens, heat inactivated specimens and body fluids other than serum.

Additional Information:

This method is approved for diagnostic testing only.  It is not approved for screening tissue donors that fall under FDA's HCT/P requirements.

Anti-hepatitis C Anti-HCV Hep C

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