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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Hepatitis B Surface Ab

Test Code(s): AHBS / HBSABI

CPT Code(s): 86706

Methodology: Chemiluminescent Immunoassay

Days Performed: Mon-Fri, dayshift.

Turnaround Time: Stat: Not available stat.
Routine: 4 hours.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red cap with yellow ring (SST)
Also Acceptable: Red top

Collection Volume: Preferred: 3 mL
Pediatrics: 1.5 mL

Sample Analyzed: Serum

Volume Required: Preferred: 1 mL
Pediatrics: 0.5 mL

Specimen Processing: Centrifuge within 2 hours.  If complete barrier has not formed, transfer cell-free serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Unacceptable Criteria: Plasma samples are not acceptable.

Stability: Ambient: 8 hours
Refrigerated: 7 days
Frozen: okay


Interpretive Guidelines:


<10 mIU/mL: Non-reactive (negative); Lack of protective immunity against HBV infection.
≥10 mIU/mL: Reactive (positive); Consistent with protective immunity against HBV infection.


Interpretation of Hepatitis B Test Result.pdf

Additional Information:

If HBcAb and HBsAg are positive with a negative HBsAb, a Hepatitis B Core Ab IgM is recommended.  If HBsAb and HBsAg are negative with a positive HBcAb, then Hepatitis B Viral DNA testing may aid in the interpretation of results.

Anti HBs Hepatitis B antibody HBsAgAb HBSA AB Antibody to hepatitis B surface antigen hep b

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