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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Hepatitis A Ab, Total

Test Code(s): HEPA / HAV

CPT Code(s): 86708

Methodology: Chemiluminescent Immunoassay

Days Performed: Daily, 24 hours.

Turnaround Time: Stat: Not available stat.
Routine: 4 hours.

Specimen Requirements

Specimen: Blood

Patient Preparation: In patients receiving therapy with high doses of biotin (>5 mg/day) no sample should be taken until at least 8 hours after the last biotin administration.

Collection Container: Preferred: Red cap with yellow ring (SST)
Also Acceptable: Red top

Collection Volume: Preferred: 4 mL
Pediatrics: 1.5 mL

Sample Analyzed: Serum

Volume Required: Preferred: 2 mL
Pediatrics: 0.5 mL

Specimen Processing: Centrifuge.  If a complete barrier has not formed, transfer cell-free serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Stability: Ambient: 12 hours
Refrigerated: 7 days
Frozen: ok; no repeat freeze/thaw cycles


Interpretive Guidelines:

Nonreactive:  Results suggest no past infection. Note: Antibody appears about 4 weeks after infection.


Reactive: A positive test for hepatitis A total antibody may indicate past infection, immunization or acute infection. Please order hepatitis A, IgM if acute hepatitis A is suspected. Hepatitis A, IgM can be ordered as an add-on test to the stored sample for a period of 6 days from the collection date of this sample. After that time, a new specimen will be required. Note on request form that test is an add-on and the collection date of specimen. Fax to UWHC Phone Center, (608) 263-0910.

Test Limitations:

This assay tests for IgG and IgM antibodies but does not differentiate between them.


Results may be affected if patient has developed heterophillic antibodies due to exposure to animal-based protein products.


The assay performance characteristics have not been established for immunocompromised or immunosuppressed patients, newborns, infants or children, cord blood, neonatal specimens, cadaver specimens, heat inactivated specimens and body fluids other than serum.


HAA Anti-HAV Hep A

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