| Test Name: |
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Gentamicin
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| Test Code(s): |
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GENT / HCGENT
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| CPT Code(s): |
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80170
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| Methodology: |
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Particle Enhanced Turbidometric Inhibition Immunoassay (PETINIA)
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| Clinical Significance: |
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Antibiotic
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| Days Performed: |
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Daily, 24 hours.
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| Turnaround Time: |
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Stat: 1 hour.
Routine: 4 hours.
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Specimen Requirements
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| Specimen: |
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Blood
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| Collection Instructions: |
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Collect specimen for peak level 30 minutes after the end of a 30 or 60 minute infusion. Collect specimen for trough level 15 - 30 minutes prior to start of infusion.
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| Collection Container: |
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Preferred: Red top
Also Acceptable:
Green top (with lithium heparin anticoagulant)
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| Collection Volume: |
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Preferred: 2 mL
Pediatrics: 0.4 mL
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| Sample Analyzed: |
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Serum or plasma
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| Volume Required: |
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Preferred: 1 mL
Pediatrics: 0.2 mL
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| Specimen Processing: |
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Centrifuge immediately. Transfer cell-free serum or plasma to plastic vial. Refrigerate.
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| Specimen Transport: |
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Transport specimen to UWHC Core Laboratory (B4/220) within 60 minutes of collection if on ice. Transport within 30 minutes if not on ice. Transport frozen serum on dry ice if coming from outreach location.
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| Unacceptable Criteria: |
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Specimens collected in red cap with yellow ring (SST) or green cap with yellow ring (PST) tubes are not acceptable.
Specimens greater than 2 hours old, uncentrifuged when received by the laboratory, are not acceptable.
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| Stability: |
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Ambient: 2 hours unspun; 8 hours spun
Refrigerated: 3 days
Frozen: 1 month
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Interpretation
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| Optimal therapeutic ranges: |
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Peak:
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4-10 mcg/mL |
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Trough:
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< 2 mcg/mL |
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| Test Limitations: |
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Aminoglycosides structurally similar to gentamicin (e.g. Sisomycin, Sagamicin and Netilmicin) may significantly cross-react with this method. Therefore, gentamicin cannot reliably be used for patients receiving these antibiotics separately or in combination with gentamicin.
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| Critical Calls: |
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View
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| Additional Information: |
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Result of "BELOW" indicates that the result is below the method detection limit of 0.2 mcg/mL. Gentamicin is an aminoglycoside antibiotic which exhibits high potency and broad spectrum antibacterial action against both gram-negative and gram-positive organisms. Patient samples which contain the drugs Sisomycin and Netilomycin will yield falsely elevated values for Gentamicin.
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