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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: FSH

Test Code(s): FSH

CPT Code(s): 83001

Methodology: Chemiluminescent Immunoassay

Request Form: UWHC Laboratory Request Form (UWH 1289220, 4004202); Write in test if not on form.

Days Performed: Mon-Fri, dayshift.

Turnaround Time: Stat: Not available stat.
Routine: Next working day.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red cap with yellow ring (SST)
Also Acceptable: Red top

Collection Volume: Preferred: 3 mL
Pediatrics: 1 mL

Sample Analyzed: Serum

Volume Required: Preferred: 1 mL
Pediatrics: 0.3 mL

Specimen Processing: Centrifuge.  If a complete barrier has not formed, transfer cell-free serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Stability: Ambient: 8 hours
Refrigerated: 48 hours
Frozen: Freeze if sample will not be assayed within 48 hours.


Reference Interval:
 Male  mIU/mL
   1 year - 11 years  0.0 - 2.8
   12 years and up  1.4 - 18.1
   1 year - 11 years  0.0 - 2.8
  12 years and up:  
    Follicular phase 2.5 - 10.2
    Midcycle peak 3.4 - 33.4
    Luteal phase 1.5 - 9.1
    Pregnant <0.3
    Postmenopausal  23.0 - 116.3



Normal infants have a transient rise in FSH to pubertal levels or higher within the first 3 months, which then declines to prepubertal values by the end of the first year.  Pediatric reference intervals from the Harriet Lane Handbook.

Test Limitations:

Results may be affected if patient has developed Human Anti-Murine Antibodies (HAMA) due to exposure to murine based products.

Additional Information:

A high dose hook effect is not seen up to 1000 mIU/mL.

Follicle Stimulating Hormone

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