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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Estradiol

Test Code(s): REST / EST

CPT Code(s): 82670

Methodology: Chemiluminescent Immunoassay

Days Performed: Daily, dayshift.

Turnaround Time: Stat: 1 hour.
Routine: 4 hours.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red cap with yellow ring (SST)
Also Acceptable: Red top

Collection Volume: Preferred: 1 mL
Pediatrics: 0.5 mL

Sample Analyzed: Serum

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.2 mL

Specimen Processing: Centrifuge.  If a complete barrier has not formed, transfer cell-free serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Stability: Ambient: 20 hours
Refrigerated: 2 days
Frozen: 6 months


Reference Interval:


  6 months - 17 years

Up to 40

  18 years and up

Up to 40

  6 months - 5 years

Up to 50

  6 years - 10 years

Up to 55

  11 years - 14 years

Up to 85

  15 years - 17 years

Up to 110

  18 years and up:  

     Follicular phase




     Luteal phase



Up to 32



Test Limitations:

Results may be affected if patient has developed heterophilic antibodies due to exposure to animal based protein products.

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