| Test Name: |
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Cortisol
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| Test Code(s): |
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CORT
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| CPT Code(s): |
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82533
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| Methodology: |
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Chemiluminescent Immunoassay
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| Days Performed: |
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Daily.
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| Turnaround Time: |
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Stat: 1 hour.
Routine: 4 hours.
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Specimen Requirements
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| Specimen: |
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Blood
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| Collection Container: |
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Preferred: Red cap with yellow ring (SST)
Also Acceptable:
Red top
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| Collection Volume: |
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Preferred: 4 mL
Pediatrics: 1 mL
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| Sample Analyzed: |
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Serum
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| Volume Required: |
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Preferred: 1 mL
Pediatrics: 0.25 mL
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| Specimen Processing: |
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Centrifuge. If a complete barrier has not formed, transfer cell-free serum to plastic vial. Refrigerate.
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| Specimen Transport: |
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Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.
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| Stability: |
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Ambient: 8 hours
Refrigerated: 48 hours
Frozen: Freeze if sample will not be assayed within 48 hours
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Interpretation
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Interpretive Guidelines:
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| AM: |
4.3-22.4 mcg/dL |
| PM: |
3.1-16.7 mcg/dL |
Applies to all ages.
Cortisol levels may be artificially elevated in patients receiving prednisolone or prednisone (which is converted to prednisolone) due to the antibody's cross reactivity with prednisolone.
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| Test Limitations: |
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Results may be affected if patient has developed heterophilic antibodies due to exposure to animal based protein products.
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