Skip to Content
UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Cocaine, Urine Screen


CPT Code(s): 80301

Test Description: Included In: Drug Screen, Urine (Stat)

Methodology: Enzyme Immunoassay

Clinical Significance: Qualitative test for benzoylecgonine.

Days Performed: Daily, 24 hours.

Turnaround Time: Stat: 1 hour. All requests processed stat.

Specimen Requirements

Specimen: Random urine

Collection Instructions: If confirmation by gas chromatography-mass spectrometry is requested on a specimen with a "screen positive" result, 10 mL of urine is needed for the confirmatory analysis.

Collection Container: Preferred: Sterile screw cap container

Collection Volume: Preferred: 25 mL
Pediatrics: 10 mL

Sample Analyzed: Urine aliquot

Volume Required: Preferred: 2 mL
Pediatrics: 0.5 mL

Specimen Processing: Refrigerate specimen.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.


This is a qualitative screening test. Results will be reported as "not detected" or "screen positive".

Test Limitations:

This is a screening test. Results are to be used for medical purposes only.

Additional Information:

A cut-off value of 300 ng/mL is used to determine whether cocaine and its metabolites are "screen positive". If screening test is reported as "screen positive", confirmation and identification can be made (at additional charge) by a reference lab using gas chromatography - mass spectrometry. Please submit an add-on order within 7 days of collection if confirmatory testing is needed.


Cocaine is rapidly metabolized in the human body to benzoylecgonine, which this method will detect. The half-life of benzoylecgonine is relatively long, on the order of 6 hours, and the metabolite may appear in the urine for days. Thus, the presence of benzoylecgonine is solid proof of past use, but the timing of the past use cannot be inferred from the urinalysis alone. (Karch, SB., Karch's Pathology of Drug Abuse, Third Edition, 2002; pg.65)


Back to Lab Test Directory Index