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American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: CK-MB and Index, if indicated

Test Code(s): MBI

CPT Code(s): 82553

Test Description: Test Component:

If a CK, Total is requested on a specimen from the same collect time, then a CK Index will be calculated and reported.  If not, only the CK-MB isoenzyme will be reported.

Included In: CK, Total and CK-MB with Index, if indicated

Methodology: Chemiluminescent Immunoassay

Request Form: UWHC Laboratory Request Form (UWH 1289220, 4004202); Write in test if not on form.

Days Performed: Daily, 24 hours.

Turnaround Time: Stat: 1 hour.
Routine: 4 hours.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Green cap with yellow ring (PST)
Also Acceptable: Green top, Red cap with yellow ring (SST), Red top

Collection Volume: Preferred: 1 mL
Pediatrics: 0.4 mL

Sample Analyzed: Plasma or serum

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.2 mL

Specimen Processing: Centrifuge.  If a complete barrier has not formed, transfer cell-free plasma/serum to plastic vial. Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Stability: Ambient: 12 hours
Refrigerated: 3 days
Frozen: 1 month


Reference Interval:

0.0 - 5.0 ng/mL


Interpretive Guidelines when used in conjunction with CK Index result:



 CKMB (ng/mL)


 Non-Acute Myocardial Infarction


 Gray Zone



 Acute Myocardial Infarction




Peak values of CK-MB greater than 5.0 ng/mL AND CK-Index greater than 4.0 are highly indicative of myocardial damage. Diagnosing myocardial injury with the aid of laboratory medicine is best done using serial samples collected over the 24-36 hours following the suspected event.

Creatine Kinase   CK-MB  CKMB

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