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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: CA 125

Test Code(s): CA125 / XCA125

CPT Code(s): 86304

Methodology: Chemiluminescent Immunoassay

Days Performed: Daily, 24 hours.

Turnaround Time: Stat: 1 hour.
Routine: 4 hours.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Green cap with yellow ring (PST)
Also Acceptable: Green top, Red cap with yellow ring (SST), Red top

Collection Volume: Preferred: 1 mL
Pediatrics: 0.5 mL

Sample Analyzed: Serum

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.15 mL

Specimen Processing: Centrifuge.  If a complete barrier has not formed, transfer cell-free serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Stability: Ambient: 2 hours on cells; 8 hours off cells
Refrigerated: 7 days
Frozen: 9 months at or below -20 C


Reference Interval:

0 days and up: 0-35 Units/mL

Test Limitations:

This method is intended to be used as an aid in the management of ovarian cancer patients with metastatic disease by monitoring the progression of regression of disease in response to treatment.  The Siemens Dimension Vista LOCI CA125 II assay is not intended for screening or diagnosis of ovarian cancer.

Results may be affected if patient has developed heterophillic antibodies due to exposure to animal based protein products.

A high dose hook effect is not seen up to 110,000 U/mL.

Additional Information:

The Siemens Dimension Vista LOCI CA125 II method is used.  Results obtained with different assay methods or kits cannot be used interchangeably.

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