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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Benzodiazepine, Urine Screen

Test Code(s): UBNZ / HCUBNZ

CPT Code(s): 80301

Test Description: Included In: Drug Screen, Urine (Stat)

Methodology: Enzyme Immunoassay

Days Performed: Daily, 24 hours.

Turnaround Time: Stat: 1 hour. All requests processed stat.

Specimen Requirements

Specimen: Random urine

Collection Instructions: If confirmation by liquid chromatography-tandem mass spectrometry is requested on a specimen with a "screen positive" result, 10 mL of urine is needed for the confirmatory analysis.

Collection Container: Preferred: Sterile screw cap container

Collection Volume: Preferred: 25 mL
Pediatrics: 10 mL

Sample Analyzed: Urine aliquot

Volume Required: Preferred: 2 mL
Pediatrics: 0.5 mL

Specimen Processing: Refrigerate specimen.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Stability: Refrigerated: Up to 24 hours
Frozen: Okay


Expected Results:

Qualitative screening test.  Results will be reported as "not detected" or "screen positive".

Test Limitations:

This is a screening test. Results are to be used for medical purposes only.

Additional Information:

A cut-off value of 200 ng/mL is used to determine whether benzodiazepine is "screen positive". If screening test is reported as "screen positive", confirmation and identification can be made (at additional charge) by a reference lab using liquid chromatography-tandem mass spectrometry. Please submit an add-on order within 7 days of collection if confirmatory testing is needed.

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