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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Acetaminophen

Test Code(s): ACTM / GM2485

CPT Code(s): 80302

Methodology: Enzymatic Hydrolysis

Clinical Significance: Analgesic

Days Performed: Daily, 24 hours.

Turnaround Time: Stat: 1 hour.
Routine: 4 hours.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red top
Also Acceptable: Green top

Collection Volume: Preferred: 1 mL
Pediatrics: 0.4 mL

Sample Analyzed: Serum or plasma

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.2 mL

Specimen Processing: Centrifuge.  Transfer cell-free serum/plasma to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Unacceptable Criteria: Specimens collected in red cap with yellow ring (SST) or green cap with yellow ring (PST) tubes are not acceptable.

Stability: Ambient: 24 hours
Refrigerated: 4 days


Generally effective therapeutic concentration:

10-30 mcg/mL



> 150 mcg/mL

Critical Calls: View

Additional Information:

If time of ingestion is unknown, samples taken 4 hours apart will give a better indication of whether toxicity exists. The concern in acetaminophen ingestion is whether:

     1. The blood concentration is increasing or decreasing

     2. The blood concentration is at a level which

         represents a threat of irreversible liver damage

     3. The potentially toxic antidote should be given.

Tylenol Datril

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