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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: AST/SGOT

Test Code(s): AST

CPT Code(s): 84450

Test Description: Included In: Comprehensive Metabolic Panel

Methodology: NADH with Pyridoxal-5 Phosphate

Days Performed: Daily, 24 hours.

Turnaround Time: Stat: 1 hour.
Routine: 4 hours.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Green cap with yellow ring (PST)
Also Acceptable: Green top, Red cap with yellow ring (SST), Red top

Collection Volume: Preferred: 1 mL
Pediatrics: 0.3 mL

Sample Analyzed: Plasma or serum

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.1 mL

Specimen Processing: Centrifuge.  Redraw specimen if hemolysis is present.  If a complete barrier has not formed, transfer cell-free plasma/serum to plastic vial.  Refrigerate.

Specimen Transport: Transport specimen to the laboratory. Transport with coolant pack if coming from outreach location.

Unacceptable Criteria: Hemolyzed specimens or whole blood specimens greater than 2 hours old when received by the laboratory are not acceptable.

Stability: Ambient: 24 hours
Refrigerated: PST - 48 hrs; if off cells or SST - 7 days
Frozen: 1 month


Reference Interval:


 Age   U/L
 0-59 days  0-80
 60 days-17 months  0-70
 18 months-9 years  0-40
 10 years and up, male  0-50
 10 years and up, female  0-40


Aspartate Aminotransferase GOT SGOT Serum Glutamic Oxalacetic Transaminase

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