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UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Vitamin B12

Test Code(s): B12

CPT Code(s): 82607

Methodology: Chemiluminescent Immunoassay

Days Performed: Daily.

Turnaround Time: Stat: Not available stat.
Routine: 4 hours.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red cap with yellow ring (SST)
Also Acceptable: Green cap with yellow ring (PST), Green top, Lavender top, Red top

Collection Volume: Preferred: 1 mL
Pediatrics: 0.7 mL

Sample Analyzed: Serum or plasma

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.25 mL

Specimen Processing: Centrifuge blood within 2 hours.  Transfer cell-free plasma/serum to plastic vial.  Refrigerate.  If transport time exceeds 48 hours, freeze plasma/serum sample.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.  Transport frozen specimen on dry ice.

Stability: Ambient: 8 hours
Refrigerated: 48 hours
Frozen: okay


Reference Interval:

0 days and up: 210-910 pg/mL

B12, Vitamin

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