Skip to Content
UW Health SMPH
American Family Children's Hospital

UW Hospital and Clinics Lab Test Directory

Test Name: Alpha-Fetoprotein

Test Code(s): AFP / XAFPTM

CPT Code(s): 82105

Methodology: Chemiluminescent Immunoassay

Request Form: UWHC Laboratory Request Form (UWH 1289220, 4004202); Write in test if not on form.

Days Performed: Mon-Fri, dayshift.

Turnaround Time: Stat: Not available stat.
Routine: Next working day.

Specimen Requirements

Specimen: Blood

Collection Container: Preferred: Red cap with yellow ring (SST)
Also Acceptable: Red top

Collection Volume: Preferred: 1 mL
Pediatrics: 0.5 mL

Sample Analyzed: Serum

Volume Required: Preferred: 0.5 mL
Pediatrics: 0.15 mL

Specimen Processing: Centrifuge.  If a complete barrier has not formed, transfer cell-free serum to plastic vial.   Refrigerate.

Specimen Transport: Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.

Stability: Ambient: 8 hours
Refrigerated: 48 hours
Frozen: Freeze if sample will not be assayed within 48 hours.


Expected Results:

8 months and up:  0.0-8.0 ng/mL


Reference range for newborns is not available but concentrations over 200,000 have been reported in newborns.  AFP gradually declines as the infant matures.  At 8 months of age, the normal AFP level of infants approaches the adult level. 

Pediatric Reference:  Serum Alpha Fetoprotein (AFP) Levels in Normal Infants, Pediatr. Res 15:50-52 1981

Test Limitations:

Results may be affected if patient has developed heterophilic antibodies due to exposure to animal based protein products.

A high dose hook effect is not seen up to 1,000,000 ng/mL.


Back to Lab Test Directory Index