| Test Name: |
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Alpha-Fetoprotein
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| Test Code(s): |
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AFP / XAFPTM
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| CPT Code(s): |
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82105
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| Methodology: |
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Chemiluminescent Immunoassay
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| Request Form: |
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UWHC Laboratory Request Form (UWH 1289220, 4004202); Write in test if not on form.
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| Days Performed: |
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Mon-Fri, dayshift.
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| Turnaround Time: |
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Stat: Not available stat.
Routine: Next working day.
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Specimen Requirements
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| Specimen: |
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Blood
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| Collection Container: |
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Preferred: Red cap with yellow ring (SST)
Also Acceptable:
Red top
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| Collection Volume: |
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Preferred: 1 mL
Pediatrics: 0.5 mL
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| Sample Analyzed: |
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Serum
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| Volume Required: |
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Preferred: 0.5 mL
Pediatrics: 0.15 mL
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| Specimen Processing: |
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Centrifuge. If a complete barrier has not formed, transfer cell-free serum to plastic vial. Refrigerate.
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| Specimen Transport: |
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Transport specimen to UWHC Core Laboratory (B4/220). Transport with coolant pack if coming from outreach location.
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| Stability: |
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Ambient: 8 hours
Refrigerated: 48 hours
Frozen: Freeze if sample will not be assayed within 48 hours.
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Interpretation
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Expected Results:
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8 months and up: 0.0-8.0 ng/mL
Reference range for newborns is not available but concentrations over 200,000 have been reported in newborns. AFP gradually declines as the infant matures. At 8 months of age, the normal AFP level of infants approaches the adult level.
Pediatric Reference: Serum Alpha Fetoprotein (AFP) Levels in Normal Infants, Pediatr. Res 15:50-52 1981
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| Test Limitations: |
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Results may be affected if patient has developed heterophilic antibodies due to exposure to animal based protein products.
A high dose hook effect is not seen up to 1,000,000 ng/mL.
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