Molecular Diagnostics | STR Engraftment Unseparated Lineages, Bone Marrow (Post-Tx)

UWHC Lab Test Details
Test NameSTR Engraftment Unseparated Lineages, Bone Marrow (Post-Tx)
Test Code(s)HCSTREBM
CPT Code(s)81267
MethodologyPCR
Clinical Information

Typically used to assess proportions of donor and recipient derived nucleated cells post stem cell transplant or in cases of suspected graft-vs-host disease.

Days PerformedOnce a week.
In-Lab Turnaround Time10 days.
Stat In-Lab Turnaround TimeNot available stat.
Collection Requirements
SpecimenBone marrow aspirate
Collection Container
Collection ContainerLavender top
Collection Instructions

Extracted nucleic acid specimens may occasionally be received in cases where no primary sample is available for testing.  All nucleic acid specimens received for testing must have been extracted in a CLIA-certified laboratory or a laboratory meeting equivalent requirements as determined by the CAP and/or the CMS.

Collection Volume1 mL
Pediatric Collection Volume1 mL
Specimen Processing Requirements
Stability
Stability Ambient

7 days

Stability Refrigerated

3 weeks

Stability Frozen

Not acceptable

Sample AnalyzedBone Marrow Aspirate
Testing Volume1 mL
Pediatric Testing Volume0.6 mL
Specimen Processing

Do not freeze
Do not centrifuge sample.

Outreach Specimen Processing

Refrigerate specimen. Do not freeze or centrifuge.

Specimen Transport

Transport specimen to Laboratory.

Interpretation

 

 >98% Donor DNA:  Predominantly Donor
 < 2% Donor DNA:  Predominantly Recipient
 2-98% Donor DNA:  Donor/Recipient Chimeric

    

Test Limitations

Test requires pre-transplant DNA samples on file in the laboratory for recipient and donor.

Additional Information

The performance characteristics of this test were validated by UWHC Clinical Laboratories. The U.S. Food and Drug Administration (FDA) has not approved or cleared this test; however, FDA approval or clearance is currently not required for clinical use of this test. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. The UWHC Clinical Laboratories is authorized under Clinical Laboratory Improvement Amendments (CLIA) to perform high-complexity testing.