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| UWHC Lab Test Details | |
|---|---|
| Test Name | Factor V (5) (Leiden) Gene Mutation by PCR |
| Test Code(s) | XFAC5, FVPCR |
| Approval Required | Testing request needs faculty review. Contact the UWHC Test Referral Office at (608)262-6388 prior to collection. |
| CPT Code(s) | 81241 |
| Methodology | Real-Time PCR followed by Melting Point Analysis |
| Clinical Information | Factor V (5) Leiden Gene Mutation Analysis is useful for confirming the diagnosis of resistance to APC as identified by functional coagulation testing. It can also be used to establish the diagnosis of resistance to APC in patients in whom functional coagulation testing may not be feasible, for example, patients on anticoagulant therapy (especially heparin) or positive for Lupus anticoagulants. Usually ordered with the Factor V (5) is a test for the Prothrombin G20210A allele, which has been associated with elevated plasma prothrombin levels and moderately increased risk of first venous thrombotic events. |
| Testing Site | University Hospital |
| Days Performed | Once a week. |
| In-Lab Turnaround Time | 10 days. |
| Stat In-Lab Turnaround Time | Not available stat. |
| Collection Requirements | |||||
|---|---|---|---|---|---|
| Specimen | Blood | ||||
| Collection Container |
| ||||
| Collection Instructions | A documented prior authorization will need to be present in Health Link before testing can be performed. The ordering source should consult the Genetic Testing at UW Health Clinical Laboratories document on U-Connect prior to ordering testing. Contact UWHC Referral office at (608) 262-6388 for questions. | ||||
| Collection Volume | 4 mL | ||||
| Pediatric Collection Volume | 2 mL | ||||
| Specimen Processing Requirements | |||||||
|---|---|---|---|---|---|---|---|
| Stability |
| ||||||
| Sample Analyzed | Whole Blood | ||||||
| Testing Volume | 2 mL | ||||||
| Pediatric Testing Volume | 1.2 mL | ||||||
| Specimen Processing | Do not centrifuge or freeze sample. | ||||||
| Outreach Specimen Processing | Refrigerate specimen. Do not freeze or centrifuge. | ||||||
| Specimen Transport | Transport specimen to Laboratory immediately. Refrigerate specimen if not delivered immediately. Transport specimen with coolant pack if coming from clinic location; avoid freezing. Specimen must be received within 7 days of collection date. | ||||||
| Interpretation | |
|---|---|
A written interpretive report is provided by the laboratory. | |
| Additional Information | This test was performed using a modification of an FDA approved method. The performance characteristics of this test were determined by the UWHC Clinical Laboratories and meet the standards for clinical testing.
The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. The UWHC Clinical Laboratories is authorized under Clinical Laboratory Improvement Amendments (CLIA) to perform high-complexity testing.
A professional fee is associated with this test.(CPT Code G0452) |
